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5h58

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Revision as of 06:52, 20 May 2020

Structural and dynamics studies of the TetR family protein, CprB from Streptomyces coelicolor in complex with its biological operator sequence

Structural highlights

5h58 is a 6 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

In Streptomycetes, tetracycline repressor family of transcription regulators (TetR-FTRs) controls various biological processes including antibiotic biosynthesis, cellular morphology and innate resistance. Here, we focus on understanding the structural basis of transcription regulation by CprB, a member of TetR-FTRs from S. coelicolor. CprB is implicated as a receptor of gamma-butyrolactones, a class of quorum sensing molecules, responsible for initiating secondary metabolic pathways. In order to understand the molecular mechanism of DNA recognition, the X-ray structure of CprB in complex with its biological relevant operator sequence was solved to a resolution of 3.95A. Furthermore, to refine and compliment the results, atomistic molecular dynamics simulations were carried out using the X-ray structure as the template. The studies reveal that CprB binds to DNA as dimer of dimers with this mode of interaction results in minimal distortion in the DNA, enabling these proteins to recognize multiple sequences with varying affinity. Another crucial finding from our simulation results was that the positively charged N-terminal arm of CprB brings extra stability to the protein-DNA complex by interacting with the minor-groove of the DNA and anchoring itself to the phosphate backbone. Corroborating electrophoretic mobility shift assay and fluorescence anisotropy experiments showed that the mutant DeltaN6-CprB exhibited about 7-8 fold reduced DNA binding. Comparison with other TetR-FTRs reveals that this strategy is also employed by over 25% of TetR-FTRs, where N-terminal anchoring mechanism is used to enhance selectivity for a particular DNA sequence.

Structural and dynamics studies of the TetR family protein, CprB from Streptomyces coelicolor in complex with its biological operator sequence.,Bhukya H, Jana AK, Sengupta N, Anand R J Struct Biol. 2017 Mar 22. pii: S1047-8477(17)30052-7. doi:, 10.1016/j.jsb.2017.03.006. PMID:28343010^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bhukya H, Jana AK, Sengupta N, Anand R. Structural and dynamics studies of the TetR family protein, CprB from Streptomyces coelicolor in complex with its biological operator sequence. J Struct Biol. 2017 Mar 22. pii: S1047-8477(17)30052-7. doi:, 10.1016/j.jsb.2017.03.006. PMID:28343010 doi:http://dx.doi.org/10.1016/j.jsb.2017.03.006

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5h58"

@@ Line 1: / Line 1: @@
 ==Structural and dynamics studies of the TetR family protein, CprB from Streptomyces coelicolor in complex with its biological operator sequence==
-<StructureSection load='5h58' size='340' side='right' caption='[[5h58]], [[Resolution|resolution]] 3.99&Aring;' scene=''>
+<StructureSection load='5h58' size='340' side='right'caption='[[5h58]], [[Resolution|resolution]] 3.99&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5h58]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H58 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5H58 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5h58]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H58 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5H58 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5h58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h58 OCA], [http://pdbe.org/5h58 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h58 RCSB], [http://www.ebi.ac.uk/pdbsum/5h58 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5h58 ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5h58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h58 OCA], [http://pdbe.org/5h58 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h58 RCSB], [http://www.ebi.ac.uk/pdbsum/5h58 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5h58 ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 19: / Line 19: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Anand, R]]
 [[Category: Bhukya, H]]

5h58

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Revision as of 06:52, 20 May 2020

Structural and dynamics studies of the TetR family protein, CprB from Streptomyces coelicolor in complex with its biological operator sequence

Structural highlights

Publication Abstract from PubMed

References

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Proteopedia Page Contributors and Editors (what is this?)

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