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5i69

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Revision as of 07:06, 3 June 2020

MBP-MamC magnetite-interaction component mutant-D70A

Structural highlights

5i69 is a 1 chain structure with sequence from Ecoli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Gene:	malE, b4034, JW3994 (ECOLI)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MALE_ECOLI] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.

Publication Abstract from PubMed

Magnetotactic bacteria are Gram-negative bacteria that navigate along geomagnetic fields using the magnetosome, an organelle that consists of a membrane-enveloped magnetic nanoparticle. Magnetite formation and its properties are controlled by a specific set of proteins. MamC is a small magnetosome-membrane protein that is known to be active in iron biomineralization but its mechanism has yet to be clarified. Here, we studied the relationship between the MamC magnetite-interaction loop (MIL) structure and its magnetite interaction using an inert biomineralization protein-MamC chimera. Our determined structure shows an alpha-helical fold for MamC-MIL with highly charged surfaces. Additionally, the MamC-MIL induces the formation of larger magnetite crystals compared to protein-free and inert biomineralization protein control experiments. We suggest that the connection between the MamC-MIL structure and the protein's charged surfaces is crucial for magnetite binding and thus for the size control of the magnetite nanoparticles.

Structure-function studies of the magnetite-biomineralizing magnetosome-associated protein MamC.,Nudelman H, Valverde-Tercedor C, Kolusheva S, Perez Gonzalez T, Widdrat M, Grimberg N, Levi H, Nelkenbaum O, Davidov G, Faivre D, Jimenez-Lopez C, Zarivach R J Struct Biol. 2016 Jun;194(3):244-52. doi: 10.1016/j.jsb.2016.03.001. Epub 2016 , Mar 10. PMID:26970040^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Nudelman H, Valverde-Tercedor C, Kolusheva S, Perez Gonzalez T, Widdrat M, Grimberg N, Levi H, Nelkenbaum O, Davidov G, Faivre D, Jimenez-Lopez C, Zarivach R. Structure-function studies of the magnetite-biomineralizing magnetosome-associated protein MamC. J Struct Biol. 2016 Jun;194(3):244-52. doi: 10.1016/j.jsb.2016.03.001. Epub 2016 , Mar 10. PMID:26970040 doi:http://dx.doi.org/10.1016/j.jsb.2016.03.001

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5i69"

@@ Line 1: / Line 1: @@
 ==MBP-MamC magnetite-interaction component mutant-D70A==
-<StructureSection load='5i69' size='340' side='right' caption='[[5i69]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
+<StructureSection load='5i69' size='340' side='right'caption='[[5i69]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5i69]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I69 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5I69 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5i69]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I69 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5I69 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MAL:MALTOSE'>MAL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MAL:MALTOSE'>MAL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5i69 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i69 OCA], [http://pdbe.org/5i69 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i69 RCSB], [http://www.ebi.ac.uk/pdbsum/5i69 PDBsum]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">malE, b4034, JW3994 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5i69 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i69 OCA], [http://pdbe.org/5i69 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i69 RCSB], [http://www.ebi.ac.uk/pdbsum/5i69 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5i69 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 22: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Ecoli]]
+[[Category: Large Structures]]
 [[Category: Davidove, G]]
 [[Category: Faivre, D]]

5i69

From Proteopedia

Revision as of 07:06, 3 June 2020

MBP-MamC magnetite-interaction component mutant-D70A

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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