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5ifg

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Revision as of 07:16, 3 June 2020

Crystal structure of HigA-HigB complex from E. Coli

Structural highlights

5ifg is a 4 chain structure with sequence from Ecoli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:
Gene:	higB, ygjN, b3083, JW3054 (ECOLI), higA, ygjM, b3082, JW3053 (ECOLI)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[HIGB_ECOLI] Toxic component of a toxin-antitoxin (TA) module. A probable translation-dependent mRNA interferase. Overexpression causes cessation of cell growth and inhibits cell proliferation via inhibition of translation; this blockage is overcome by subsequent expression of antitoxin HigA. Overexpression causes cleavage of a number of mRNAs in a translation-dependent fashion, suggesting this is an mRNA interferase.^[1] [HIGA_ECOLI] Antitoxin component of a toxin-antitoxin (TA) module. Functions as an mRNA interferase antitoxin; overexpression prevents HigB-mediated cessation of cell growth and inhibition of cell proliferation.^[2]

Publication Abstract from PubMed

The toxin-antitoxin system is ubiquitously existed in bacteria and archaea, performing a wide variety of functions modulating cell fitness in response to environmental cues. In this report, we solved the crystal structure of the toxin-antitoxin HigBA complex from E. coli K-12 to 2.7 A resolution. The crystal structure of the HigBA complex displays a hetero-tetramer (HigBA)2 form comprised by two HigB and two HigA subunits. Each toxin HigB resumes a microbial RNase T1 fold, characteristic of a three antiparallel beta-sheet core shielded by a few alpha-helices at either side. Each antitoxin HigA composed of all alpha-helices resembles a "C"-shaped clamp nicely encompassing a HigB in the (HigBA)2 complex. Two HigA monomers dimerize at their N-terminal domain. We showed that HigA helix alpha1 was essential for HigA dimerization and the hetero-tetramer (HigBA)2 formation, but not for a hetero-dimeric HigBA formation. HigA dimerization mediated by helix alpha1 was dispensable for DNA-binding, as a heterodimeric HigBA complex still bound to the higBA operator in vitro. The HigA C-terminal domain with a helix-turn-helix fold was essential for DNA binding. We also defined two palindromes in higBA operator specifically recognized by HigA and HigBA in vitro.

Structural insight into the E. coli HigBA complex.,Yang J, Zhou K, Liu P, Dong Y, Gao Z, Zhang J, Liu Q Biochem Biophys Res Commun. 2016 Sep 30;478(4):1521-7. doi:, 10.1016/j.bbrc.2016.08.131. Epub 2016 Sep 4. PMID:27601326^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Christensen-Dalsgaard M, Jorgensen MG, Gerdes K. Three new RelE-homologous mRNA interferases of Escherichia coli differentially induced by environmental stresses. Mol Microbiol. 2010 Jan;75(2):333-48. doi: 10.1111/j.1365-2958.2009.06969.x. Epub, 2009 Nov 25. PMID:19943910 doi:http://dx.doi.org/10.1111/j.1365-2958.2009.06969.x
↑ Christensen-Dalsgaard M, Jorgensen MG, Gerdes K. Three new RelE-homologous mRNA interferases of Escherichia coli differentially induced by environmental stresses. Mol Microbiol. 2010 Jan;75(2):333-48. doi: 10.1111/j.1365-2958.2009.06969.x. Epub, 2009 Nov 25. PMID:19943910 doi:http://dx.doi.org/10.1111/j.1365-2958.2009.06969.x
↑ Yang J, Zhou K, Liu P, Dong Y, Gao Z, Zhang J, Liu Q. Structural insight into the E. coli HigBA complex. Biochem Biophys Res Commun. 2016 Sep 30;478(4):1521-7. doi:, 10.1016/j.bbrc.2016.08.131. Epub 2016 Sep 4. PMID:27601326 doi:http://dx.doi.org/10.1016/j.bbrc.2016.08.131

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5ifg"

@@ Line 1: / Line 1: @@
 ==Crystal structure of HigA-HigB complex from E. Coli==
-<StructureSection load='5ifg' size='340' side='right' caption='[[5ifg]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
+<StructureSection load='5ifg' size='340' side='right'caption='[[5ifg]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5ifg]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IFG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IFG FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5ifg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IFG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5IFG FirstGlance]. <br>
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ifg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ifg OCA], [http://pdbe.org/5ifg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ifg RCSB], [http://www.ebi.ac.uk/pdbsum/5ifg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ifg ProSAT]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">higB, ygjN, b3083, JW3054 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI]), higA, ygjM, b3082, JW3053 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5ifg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ifg OCA], [http://pdbe.org/5ifg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ifg RCSB], [http://www.ebi.ac.uk/pdbsum/5ifg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ifg ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 22: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Ecoli]]
+[[Category: Large Structures]]
 [[Category: Dong, Y H]]
 [[Category: Gao, z Q]]

5ifg

From Proteopedia

Revision as of 07:16, 3 June 2020

Crystal structure of HigA-HigB complex from E. Coli

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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