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6sw2
From Proteopedia
(Difference between revisions)
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==Crystal Structure of P. aeruginosa PqsL in complex with 2-aminobenzoylacetate== | ==Crystal Structure of P. aeruginosa PqsL in complex with 2-aminobenzoylacetate== | ||
| - | <StructureSection load='6sw2' size='340' side='right'caption='[[6sw2]]' scene=''> | + | <StructureSection load='6sw2' size='340' side='right'caption='[[6sw2]], [[Resolution|resolution]] 1.70Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SW2 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SW2 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6sw2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SW2 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SW2 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6sw2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sw2 OCA], [http://pdbe.org/6sw2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sw2 RCSB], [http://www.ebi.ac.uk/pdbsum/6sw2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sw2 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=61M:3-(2-AMINOPHENYL)-3-OXOPROPANOIC+ACID'>61M</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> |
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pqsL, PA4190 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6sw2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sw2 OCA], [http://pdbe.org/6sw2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sw2 RCSB], [http://www.ebi.ac.uk/pdbsum/6sw2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sw2 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Light-dependent or light-stimulated catalysis provides a multitude of perspectives for implementation in technological or biomedical applications. Despite substantial progress made in the field of photobiocatalysis, the number of usable light-responsive enzymes is still very limited. Flavoproteins have exceptional potential for photocatalytic applications because the name-giving cofactor intrinsically features light-dependent reactivity, undergoing photoreduction with a variety of organic electron donors. However, in the vast majority of these enzymes, photoreactivity of the enzyme-bound flavin is limited or even suppressed. Here, we present a flavoprotein monooxygenase in which catalytic activity is controllable by blue light illumination. The reaction depends on the presence of nicotinamide nucleotide-type electron donors, which do not support the reaction in the absence of light. Employing various experimental approaches, we demonstrate that catalysis depends on a protein-mediated photoreduction of the flavin cofactor, which proceeds via a radical mechanism and a transient semiquinone intermediate. | ||
| + | |||
| + | Photoinduced monooxygenation involving NAD(P)H-FAD sequential single-electron transfer.,Ernst S, Rovida S, Mattevi A, Fetzner S, Drees SL Nat Commun. 2020 May 25;11(1):2600. doi: 10.1038/s41467-020-16450-y. PMID:32451409<ref>PMID:32451409</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6sw2" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Mattevi, A | + | [[Category: Pseae]] |
| + | [[Category: Mattevi, A]] | ||
| + | [[Category: Rovida, S]] | ||
| + | [[Category: Antibiotic]] | ||
| + | [[Category: Biosynthetic pathway]] | ||
| + | [[Category: Fad]] | ||
| + | [[Category: Oxidoreductase]] | ||
| + | [[Category: Photocatalysis]] | ||
Revision as of 06:41, 10 June 2020
Crystal Structure of P. aeruginosa PqsL in complex with 2-aminobenzoylacetate
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