This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

2rpv

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 11:04, 17 June 2020

Solution Structure of GB1 with LBT probe

Structural highlights

2rpv is a 1 chain structure with sequence from Strsg. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SPG1_STRSG] Binds to the constant Fc region of IgG with high affinity.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Paramagnetic lanthanide ions fixed in a protein frame induce several paramagnetic effects such as pseudo-contact shifts and residual dipolar couplings. These effects provide long-range distance and angular information for proteins and, therefore, are valuable in protein structural analysis. However, until recently this approach had been restricted to metal-binding proteins, but now it has become applicable to non-metalloproteins through the use of a lanthanide-binding tag. Here we report a lanthanide-binding peptide tag anchored via two points to the target proteins. Compared to conventional single-point attached tags, the two-point linked tag provides two to threefold stronger anisotropic effects. Though there is slight residual mobility of the lanthanide-binding tag, the present tag provides a higher anisotropic paramagnetic effect.

Two-point anchoring of a lanthanide-binding peptide to a target protein enhances the paramagnetic anisotropic effect.,Saio T, Ogura K, Yokochi M, Kobashigawa Y, Inagaki F J Biomol NMR. 2009 Jul;44(3):157-66. Epub 2009 May 26. PMID:19468839^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Saio T, Ogura K, Yokochi M, Kobashigawa Y, Inagaki F. Two-point anchoring of a lanthanide-binding peptide to a target protein enhances the paramagnetic anisotropic effect. J Biomol NMR. 2009 Jul;44(3):157-66. Epub 2009 May 26. PMID:19468839 doi:10.1007/s10858-009-9325-z

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2rpv"

@@ Line 1: / Line 1: @@
 ==Solution Structure of GB1 with LBT probe==
-<StructureSection load='2rpv' size='340' side='right' caption='[[2rpv]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2rpv' size='340' side='right'caption='[[2rpv]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2rpv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strsg Strsg]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RPV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RPV FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2rpv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strsg Strsg]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RPV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2RPV FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LA:LANTHANUM+(III)+ION'>LA</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LA:LANTHANUM+(III)+ION'>LA</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rpv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rpv OCA], [http://pdbe.org/2rpv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rpv RCSB], [http://www.ebi.ac.uk/pdbsum/2rpv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2rpv ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2rpv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rpv OCA], [http://pdbe.org/2rpv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rpv RCSB], [http://www.ebi.ac.uk/pdbsum/2rpv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2rpv ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 35: / Line 35: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Strsg]]
 [[Category: Inagaki, F]]

2rpv

From Proteopedia

Revision as of 11:04, 17 June 2020

Solution Structure of GB1 with LBT probe

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox