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1bl3

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[[Image:1bl3.gif|left|200px]]
[[Image:1bl3.gif|left|200px]]
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{{Structure
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|PDB= 1bl3 |SIZE=350|CAPTION= <scene name='initialview01'>1bl3</scene>, resolution 2.00&Aring;
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|SITE= <scene name='pdbsite=ACT:Active+Site.+Mg-Binding+Site'>ACT</scene>
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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{{STRUCTURE_1bl3| PDB=1bl3 | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bl3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bl3 OCA], [http://www.ebi.ac.uk/pdbsum/1bl3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1bl3 RCSB]</span>
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'''CATALYTIC DOMAIN OF HIV-1 INTEGRASE'''
'''CATALYTIC DOMAIN OF HIV-1 INTEGRASE'''
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[[Category: Mikol, V.]]
[[Category: Mikol, V.]]
[[Category: Zhou-Liu, Q.]]
[[Category: Zhou-Liu, Q.]]
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[[Category: aid]]
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[[Category: Aid]]
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[[Category: dna binding (viral)]]
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[[Category: Dna integration]]
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[[Category: dna integration]]
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[[Category: Endonuclease]]
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[[Category: endonuclease]]
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[[Category: Hydrolase]]
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[[Category: hydrolase]]
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[[Category: Polynucleotidyl transferase]]
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[[Category: polynucleotidyl transferase]]
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[[Category: Polyprotein]]
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[[Category: polyprotein]]
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Revision as of 08:39, 2 May 2008

Template:STRUCTURE 1bl3

CATALYTIC DOMAIN OF HIV-1 INTEGRASE


Overview

Human immunodeficiency virus (HIV) integrase is the enzyme responsible for insertion of a DNA copy of the viral genome into host DNA, an essential step in the replication cycle of HIV. HIV-1 integrase comprises three functional and structural domains: an N-terminal zinc-binding domain, a catalytic core domain and a C-terminal DNA-binding domain. The catalytic core domain with the F185H mutation has been crystallized without sodium cacodylate in a new crystal form, free and complexed with the catalytic metal Mg2+. The structures have been determined and refined to about 2.2 A. Unlike the previously reported structures, the three active-site carboxylate residues (D,D-35-E motif) are well ordered and both aspartate residues delineate a proper metal-binding site. Comparison of the active binding site of this domain with that of other members from the polynucleotidyl transferases superfamily shows a high level of similarity, providing a confident template for the design of antiviral agents.

About this Structure

1BL3 is a Single protein structure of sequence from Human immunodeficiency virus 1. Full crystallographic information is available from OCA.

Reference

Crystal structures of the catalytic domain of HIV-1 integrase free and complexed with its metal cofactor: high level of similarity of the active site with other viral integrases., Maignan S, Guilloteau JP, Zhou-Liu Q, Clement-Mella C, Mikol V, J Mol Biol. 1998 Sep 18;282(2):359-68. PMID:9735293 Page seeded by OCA on Fri May 2 11:39:12 2008

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