2vpp

From Proteopedia

(Difference between revisions)

Revision as of 08:18, 25 June 2020

Drosophila melanogaster deoxyribonucleoside kinase successfully activates gemcitabine in transduced cancer cell lines

Structural highlights

2vpp is a 2 chain structure with sequence from Drome. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	1zm7, 2vp5, 1zmx, 2vp4, 1j90, 2vp0, 2jcs, 1ot3, 2vp6, 1oe0, 2vp2, 2vp9
Activity:	Deoxynucleoside kinase, with EC number 2.7.1.145
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[DNK_DROME] Deoxyribonucleoside kinase that has a broad specificity phosphorylating thymidine, deoxyadenosine, deoxycytidine and deoxyguanosine. Specificity is higher for pyrimidine nucleosides. Several anti-viral and anti-cancer nucleoside analogs are also efficiently phosphorylated.^[1] ^[2] ^[3]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Drosophila melanogaster multisubstrate deoxyribonucleoside kinase (Dm-dNK) can additionally sensitize human cancer cell lines towards the anti-cancer drug gemcitabine. We show that this property is based on the Dm-dNK ability to efficiently phosphorylate gemcitabine. The 2.2A resolution structure of Dm-dNK in complex with gemcitabine shows that the residues Tyr70 and Arg105 play a crucial role in the firm positioning of gemcitabine by extra interactions made by the fluoride atoms. This explains why gemcitabine is a good substrate for Dm-dNK.

Drosophila melanogaster deoxyribonucleoside kinase activates gemcitabine.,Knecht W, Mikkelsen NE, Clausen AR, Willer M, Eklund H, Gojkovic Z, Piskur J Biochem Biophys Res Commun. 2009 May 1;382(2):430-3. Epub 2009 Mar 13. PMID:19285960^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Johansson M, van Rompay AR, Degreve B, Balzarini J, Karlsson A. Cloning and characterization of the multisubstrate deoxyribonucleoside kinase of Drosophila melanogaster. J Biol Chem. 1999 Aug 20;274(34):23814-9. PMID:10446143
↑ Munch-Petersen B, Knecht W, Lenz C, Sondergaard L, Piskur J. Functional expression of a multisubstrate deoxyribonucleoside kinase from Drosophila melanogaster and its C-terminal deletion mutants. J Biol Chem. 2000 Mar 3;275(9):6673-9. PMID:10692477
↑ Welin M, Skovgaard T, Knecht W, Zhu C, Berenstein D, Munch-Petersen B, Piskur J, Eklund H. Structural basis for the changed substrate specificity of Drosophila melanogaster deoxyribonucleoside kinase mutant N64D. FEBS J. 2005 Jul;272(14):3733-42. PMID:16008571 doi:10.1111/j.1742-4658.2005.04803.x
↑ Knecht W, Mikkelsen NE, Clausen AR, Willer M, Eklund H, Gojkovic Z, Piskur J. Drosophila melanogaster deoxyribonucleoside kinase activates gemcitabine. Biochem Biophys Res Commun. 2009 May 1;382(2):430-3. Epub 2009 Mar 13. PMID:19285960 doi:http://dx.doi.org/10.1016/j.bbrc.2009.03.041

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2vpp"

@@ Line 1: / Line 1: @@
 ==Drosophila melanogaster deoxyribonucleoside kinase successfully activates gemcitabine in transduced cancer cell lines==
-<StructureSection load='2vpp' size='340' side='right' caption='[[2vpp]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+<StructureSection load='2vpp' size='340' side='right'caption='[[2vpp]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2vpp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VPP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VPP FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2vpp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VPP OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2VPP FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GEO:GEMCITABINE'>GEO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GEO:GEMCITABINE'>GEO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1zm7|1zm7]], [[2vp5|2vp5]], [[1zmx|1zmx]], [[2vp4|2vp4]], [[1j90|1j90]], [[2vp0|2vp0]], [[2jcs|2jcs]], [[1ot3|1ot3]], [[2vp6|2vp6]], [[1oe0|1oe0]], [[2vp2|2vp2]], [[2vp9|2vp9]]</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxynucleoside_kinase Deoxynucleoside kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.145 2.7.1.145] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vpp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vpp OCA], [http://pdbe.org/2vpp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vpp RCSB], [http://www.ebi.ac.uk/pdbsum/2vpp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vpp ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2vpp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vpp OCA], [http://pdbe.org/2vpp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vpp RCSB], [http://www.ebi.ac.uk/pdbsum/2vpp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vpp ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 36: / Line 36: @@
 [[Category: Deoxynucleoside kinase]]
 [[Category: Drome]]
+[[Category: Large Structures]]
 [[Category: Clausen, A]]
 [[Category: Gojkovic, Z]]

2vpp

From Proteopedia

Revision as of 08:18, 25 June 2020

Drosophila melanogaster deoxyribonucleoside kinase successfully activates gemcitabine in transduced cancer cell lines

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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