2vqz

From Proteopedia

(Difference between revisions)

Revision as of 08:23, 25 June 2020

Structure of the cap-binding domain of influenza virus polymerase subunit PB2 with bound m7GTP

Structural highlights

2vqz is a 5 chain structure with sequence from 9infa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
NonStd Res:
Related:	2jdq
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PB2_I75A3] Involved in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. Binds the cap of the target pre-RNA which is subsequently cleaved by PB1. May play a role in genome replication (By similarity).

Publication Abstract from PubMed

Influenza virus mRNAs are synthesized by the trimeric viral polymerase using short capped primers obtained by a 'cap-snatching' mechanism. The polymerase PB2 subunit binds the 5' cap of host pre-mRNAs, which are cleaved after 10-13 nucleotides by the PB1 subunit. Using a library-screening method, we identified an independently folded domain of PB2 that has specific cap binding activity. The X-ray structure of the domain with bound cap analog m(7)GTP at 2.3-A resolution reveals a previously unknown fold and a mode of ligand binding that is similar to, but distinct from, other cap binding proteins. Binding and functional studies with point mutants confirm that the identified site is essential for cap binding in vitro and cap-dependent transcription in vivo by the trimeric polymerase complex. These findings clarify the nature of the cap binding site in PB2 and will allow efficient structure-based design of new anti-influenza compounds inhibiting viral transcription.

The structural basis for cap binding by influenza virus polymerase subunit PB2.,Guilligay D, Tarendeau F, Resa-Infante P, Coloma R, Crepin T, Sehr P, Lewis J, Ruigrok RW, Ortin J, Hart DJ, Cusack S Nat Struct Mol Biol. 2008 May;15(5):500-6. Epub 2008 May 4. PMID:18454157^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Guilligay D, Tarendeau F, Resa-Infante P, Coloma R, Crepin T, Sehr P, Lewis J, Ruigrok RW, Ortin J, Hart DJ, Cusack S. The structural basis for cap binding by influenza virus polymerase subunit PB2. Nat Struct Mol Biol. 2008 May;15(5):500-6. Epub 2008 May 4. PMID:18454157 doi:10.1038/nsmb.1421

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2vqz"

@@ Line 1: / Line 1: @@
 ==Structure of the cap-binding domain of influenza virus polymerase subunit PB2 with bound m7GTP==
-<StructureSection load='2vqz' size='340' side='right' caption='[[2vqz]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+<StructureSection load='2vqz' size='340' side='right'caption='[[2vqz]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2vqz]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/9infa 9infa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VQZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VQZ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2vqz]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/9infa 9infa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VQZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2VQZ FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MGT:7N-METHYL-8-HYDROGUANOSINE-5-TRIPHOSPHATE'>MGT</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MGT:7N-METHYL-8-HYDROGUANOSINE-5-TRIPHOSPHATE'>MGT</scene></td></tr>
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2jdq|2jdq]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vqz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vqz OCA], [http://pdbe.org/2vqz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vqz RCSB], [http://www.ebi.ac.uk/pdbsum/2vqz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vqz ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2vqz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vqz OCA], [http://pdbe.org/2vqz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vqz RCSB], [http://www.ebi.ac.uk/pdbsum/2vqz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vqz ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 22: / Line 22: @@
 ==See Also==
-*[[RNA polymerase|RNA polymerase]]
+*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Coloma, R]]
 [[Category: Crepin, T]]

2vqz

From Proteopedia

Revision as of 08:23, 25 June 2020

Structure of the cap-binding domain of influenza virus polymerase subunit PB2 with bound m7GTP

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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