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2wiv

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Revision as of 08:47, 1 July 2020

Cytochrome-P450 XplA heme domain P21

Structural highlights

2wiv is a 2 chain structure with sequence from "prescottella"_jones_et_al._2013 "prescottella" jones et al. 2013. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	2wiy
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

XplA is a cytochrome P450 of unique structural organization, consisting of a heme-domain that is C-terminally fused to its native flavodoxin redox partner. XplA, along with flavodoxin reductase XplB, has been shown to catalyze the breakdown of the nitramine explosive and pollutant hexahydro-1,3,5-trinitro-1,3,5-triazine (royal demolition explosive) by reductive denitration. The structure of the heme domain of XplA (XplA-heme) has been solved in two crystal forms: as a dimer in space group P2(1) to a resolution of 1.9 A and as a monomer in space group P2(1)2(1)2 to a resolution of 1.5 A, with the ligand imidazole bound at the heme iron. Although it shares the overall fold of cytochromes P450 of known structure, XplA-heme is unusual in that the kinked I-helix that traverses the distal face of the heme is broken by Met-394 and Ala-395 in place of the well conserved Asp/Glu plus Thr/Ser, important in oxidative P450s for the scission of the dioxygen bond prior to substrate oxygenation. The heme environment of XplA-heme is hydrophobic, featuring a cluster of three methionines above the heme, including Met-394. Imidazole was observed bound to the heme iron and is in close proximity to the side chain of Gln-438, which is situated over the distal face of the heme. Imidazole is also hydrogen-bonded to a water molecule that sits in place of the threonine side-chain hydroxyl exemplified by Thr-252 in Cyt-P450cam. Both Gln-438 --> Ala and Ala-395 --> Thr mutants of XplA-heme displayed markedly reduced activity compared with the wild type for royal demolition explosive degradation when combined with surrogate electron donors.

The 1.5-A structure of XplA-heme, an unusual cytochrome P450 heme domain that catalyzes reductive biotransformation of royal demolition explosive.,Sabbadin F, Jackson R, Haider K, Tampi G, Turkenburg JP, Hart S, Bruce NC, Grogan G J Biol Chem. 2009 Oct 9;284(41):28467-75. Epub 2009 Aug 19. PMID:19692330^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sabbadin F, Jackson R, Haider K, Tampi G, Turkenburg JP, Hart S, Bruce NC, Grogan G. The 1.5-A structure of XplA-heme, an unusual cytochrome P450 heme domain that catalyzes reductive biotransformation of royal demolition explosive. J Biol Chem. 2009 Oct 9;284(41):28467-75. Epub 2009 Aug 19. PMID:19692330 doi:10.1074/jbc.M109.031559

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2wiv"

@@ Line 1: / Line 1: @@
 ==Cytochrome-P450 XplA heme domain P21==
-<StructureSection load='2wiv' size='340' side='right' caption='[[2wiv]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+<StructureSection load='2wiv' size='340' side='right'caption='[[2wiv]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2wiv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"prescottella"_jones_et_al._2013 "prescottella" jones et al. 2013]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WIV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WIV FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2wiv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"prescottella"_jones_et_al._2013 "prescottella" jones et al. 2013]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WIV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2WIV FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2wiy|2wiy]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wiv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wiv OCA], [http://pdbe.org/2wiv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wiv RCSB], [http://www.ebi.ac.uk/pdbsum/2wiv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wiv ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2wiv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wiv OCA], [http://pdbe.org/2wiv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wiv RCSB], [http://www.ebi.ac.uk/pdbsum/2wiv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wiv ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 32: / Line 32: @@
 </StructureSection>
 [[Category: Prescottella jones et al. 2013]]
+[[Category: Large Structures]]
 [[Category: Bruce, N C]]
 [[Category: Grogan, G]]

2wiv

From Proteopedia

Revision as of 08:47, 1 July 2020

Cytochrome-P450 XplA heme domain P21

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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