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2x79

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Revision as of 15:06, 8 July 2020

Inward facing conformation of Mhp1

Structural highlights

2x79 is a 1 chain structure with sequence from "microbacterium_liquefaciens"_orla-jensen_1919 "microbacterium liquefaciens" orla-jensen 1919. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The structure of the sodium-benzylhydantoin transport protein Mhp1 from Microbacterium liquefaciens comprises a five-helix inverted repeat, which is widespread among secondary transporters. Here, we report the crystal structure of an inward-facing conformation of Mhp1 at 3.8 angstroms resolution, complementing its previously described structures in outward-facing and occluded states. From analyses of the three structures and molecular dynamics simulations, we propose a mechanism for the transport cycle in Mhp1. Switching from the outward- to the inward-facing state, to effect the inward release of sodium and benzylhydantoin, is primarily achieved by a rigid body movement of transmembrane helices 3, 4, 8, and 9 relative to the rest of the protein. This forms the basis of an alternating access mechanism applicable to many transporters of this emerging superfamily.

Molecular basis of alternating access membrane transport by the sodium-hydantoin transporter Mhp1.,Shimamura T, Weyand S, Beckstein O, Rutherford NG, Hadden JM, Sharples D, Sansom MS, Iwata S, Henderson PJ, Cameron AD Science. 2010 Apr 23;328(5977):470-3. PMID:20413494^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Shimamura T, Weyand S, Beckstein O, Rutherford NG, Hadden JM, Sharples D, Sansom MS, Iwata S, Henderson PJ, Cameron AD. Molecular basis of alternating access membrane transport by the sodium-hydantoin transporter Mhp1. Science. 2010 Apr 23;328(5977):470-3. PMID:20413494 doi:328/5977/470

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2x79"

@@ Line 1: / Line 1: @@
 ==Inward facing conformation of Mhp1==
-<StructureSection load='2x79' size='340' side='right' caption='[[2x79]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
+<StructureSection load='2x79' size='340' side='right'caption='[[2x79]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2x79]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"microbacterium_liquefaciens"_orla-jensen_1919 "microbacterium liquefaciens" orla-jensen 1919]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X79 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2X79 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2x79]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"microbacterium_liquefaciens"_orla-jensen_1919 "microbacterium liquefaciens" orla-jensen 1919]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X79 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2X79 FirstGlance]. <br>
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2x79 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x79 OCA], [http://pdbe.org/2x79 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2x79 RCSB], [http://www.ebi.ac.uk/pdbsum/2x79 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2x79 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2x79 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x79 OCA], [http://pdbe.org/2x79 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2x79 RCSB], [http://www.ebi.ac.uk/pdbsum/2x79 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2x79 ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 31: / Line 31: @@
 </StructureSection>
 [[Category: Microbacterium liquefaciens orla-jensen 1919]]
+[[Category: Large Structures]]
 [[Category: Beckstein, O]]
 [[Category: Cameron, A D]]

2x79

From Proteopedia

Revision as of 15:06, 8 July 2020

Inward facing conformation of Mhp1

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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