6sjj
From Proteopedia
(Difference between revisions)
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==A new modulated crystal structure of ANS complex of St John's wort Hyp-1 protein with 36 protein molecules in the asymmetric unit of the supercell== | ==A new modulated crystal structure of ANS complex of St John's wort Hyp-1 protein with 36 protein molecules in the asymmetric unit of the supercell== | ||
| - | <StructureSection load='6sjj' size='340' side='right'caption='[[6sjj]]' scene=''> | + | <StructureSection load='6sjj' size='340' side='right'caption='[[6sjj]], [[Resolution|resolution]] 2.30Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SJJ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SJJ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6sjj]] is a 36 chain structure with sequence from [http://en.wikipedia.org/wiki/Hyppe Hyppe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SJJ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SJJ FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6sjj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sjj OCA], [http://pdbe.org/6sjj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sjj RCSB], [http://www.ebi.ac.uk/pdbsum/6sjj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sjj ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2AN:8-ANILINO-1-NAPHTHALENE+SULFONATE'>2AN</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PR10.1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=65561 HYPPE])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6sjj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sjj OCA], [http://pdbe.org/6sjj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sjj RCSB], [http://www.ebi.ac.uk/pdbsum/6sjj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sjj ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Superstructure modulation, with violation of the strict short-range periodic order of consecutive crystal unit cells, is well known in small-molecule crystallography but is rarely reported for macromolecular crystals. To date, one modulated macromolecular crystal structure has been successfully determined and refined for a pathogenesis-related class 10 protein from Hypericum perforatum (Hyp-1) crystallized as a complex with 8-anilinonaphthalene-1-sulfonate (ANS) [Sliwiak et al. (2015), Acta Cryst. D71, 829-843]. The commensurate modulation in that case was interpreted in a supercell with sevenfold expansion along c. When crystallized in the additional presence of melatonin, the Hyp-1-ANS complex formed crystals with a different pattern of structure modulation, in which the supercell shows a ninefold expansion of c, manifested in the diffraction pattern by a wave of reflection-intensity modulation with crests at l = 9n and l = 9n +/- 4. Despite complicated tetartohedral twinning, the structure has been successfully determined and refined to 2.3 A resolution using a description in a ninefold-expanded supercell, with 36 independent Hyp-1 chains and 156 ANS ligands populating the three internal (95 ligands) and five interstitial (61 ligands) binding sites. The commensurate superstructures and ligand-binding sites of the two crystal structures are compared, with a discussion of the effect of melatonin on the co-crystallization process. | ||
| + | |||
| + | A new modulated crystal structure of the ANS complex of the St John's wort Hyp-1 protein with 36 protein molecules in the asymmetric unit of the supercell.,Smietanska J, Sliwiak J, Gilski M, Dauter Z, Strzalka R, Wolny J, Jaskolski M Acta Crystallogr D Struct Biol. 2020 Jul 1;76(Pt 7):653-667. doi:, 10.1107/S2059798320006841. Epub 2020 Jun 17. PMID:32627738<ref>PMID:32627738</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6sjj" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Hyppe]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Dauter Z]] | + | [[Category: Dauter, Z]] |
| - | [[Category: Gilski M]] | + | [[Category: Gilski, M]] |
| - | [[Category: Jaskolski M]] | + | [[Category: Jaskolski, M]] |
| - | [[Category: Sliwiak J]] | + | [[Category: Sliwiak, J]] |
| - | [[Category: Smietanska J]] | + | [[Category: Smietanska, J]] |
| - | [[Category: Strzalka R]] | + | [[Category: Strzalka, R]] |
| - | [[Category: Wolny J]] | + | [[Category: Wolny, J]] |
| + | [[Category: Commensurately modulated superstructure]] | ||
| + | [[Category: Cytokinin]] | ||
| + | [[Category: Depression]] | ||
| + | [[Category: Glycine-rich loop]] | ||
| + | [[Category: Hydrophobic cavity]] | ||
| + | [[Category: Hypericin]] | ||
| + | [[Category: Pathogenesis-related protein]] | ||
| + | [[Category: Phytohormone binding]] | ||
| + | [[Category: Plant defense]] | ||
| + | [[Category: Plant hormone binding]] | ||
| + | [[Category: Plant protein]] | ||
| + | [[Category: Pr-10 fold]] | ||
| + | [[Category: Pr-10 protein]] | ||
| + | [[Category: Tetartohedral twinning]] | ||
Revision as of 11:28, 22 July 2020
A new modulated crystal structure of ANS complex of St John's wort Hyp-1 protein with 36 protein molecules in the asymmetric unit of the supercell
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Categories: Hyppe | Large Structures | Dauter, Z | Gilski, M | Jaskolski, M | Sliwiak, J | Smietanska, J | Strzalka, R | Wolny, J | Commensurately modulated superstructure | Cytokinin | Depression | Glycine-rich loop | Hydrophobic cavity | Hypericin | Pathogenesis-related protein | Phytohormone binding | Plant defense | Plant hormone binding | Plant protein | Pr-10 fold | Pr-10 protein | Tetartohedral twinning
