Galactose mutarotase
From Proteopedia
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| - | <StructureSection load='1ns0' size='350' side='right' caption='Structure of galactose mutarotase complex with α-D-galactose and Na+ ion (purple) (PDB entry [[1ns0]])' scene=''> | + | <StructureSection load='1ns0' size='350' side='right' caption='Structure of galactose mutarotase complex with α-D-galactose and Na+ ion (purple) (PDB entry [[1ns0]])' scene='57/572275/Cv/1'> |
| + | __TOC__ | ||
== Function == | == Function == | ||
'''Galactose mutarotase''' (GalM) reversibly converts α-lactose to β-lactose. GalM catalyzes the first step in galactose metabolism<ref>PMID:12717027</ref>. | '''Galactose mutarotase''' (GalM) reversibly converts α-lactose to β-lactose. GalM catalyzes the first step in galactose metabolism<ref>PMID:12717027</ref>. | ||
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| + | == Structural highlights == | ||
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| + | From extensive study of mutations in ''Lactococcus lactis'' GalM complexes with various sugars it was found that residues H170 and E304 are critical for the active site binding. | ||
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| + | <scene name='57/572275/Cv/7'>Whole active site</scene>. Water molecules shown as red spheres. | ||
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| + | <scene name='57/572275/Cv/8'>Na coordination site</scene>. | ||
== 3D structures of galactose mutarotase == | == 3D structures of galactose mutarotase == | ||
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | ||
| + | [[1snz]] – hGalM – human<br /> | ||
| + | [[1so0]] - hGalM (mutant) + α-D-galactose <br /> | ||
[[1l7j]] – LlGalM – ''Lactococcus lactis'' <br /> | [[1l7j]] – LlGalM – ''Lactococcus lactis'' <br /> | ||
[[1l7k]], [[1ns0]], [[1ns2]], [[1ns8]], [[1nsu]], [[1nsx]] – LlGalM (mutant) + α-D-galactose <br /> | [[1l7k]], [[1ns0]], [[1ns2]], [[1ns8]], [[1nsu]], [[1nsx]] – LlGalM (mutant) + α-D-galactose <br /> | ||
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[[1nsm]] – LlGalM (mutant) + α-D-galactose + β-D-galactose <br /> | [[1nsm]] – LlGalM (mutant) + α-D-galactose + β-D-galactose <br /> | ||
[[1lur]] – GalM – ''Caenorhabditis elegans''<br /> | [[1lur]] – GalM – ''Caenorhabditis elegans''<br /> | ||
| - | [[ | + | [[3mwx]], [[4bze]] - BsGalM – ''Bacillus subtilis''<br /> |
| - | [[ | + | [[4bzf]] - BsGalM + trehalose<br /> |
| - | [[ | + | [[4bzg]] - BsGalM + maltose<br /> |
| + | [[4bzh]] - BsGalM + trehalose + maltose<br /> | ||
[[3nre]] – GalM – ''Escherichia coli''<br /> | [[3nre]] – GalM – ''Escherichia coli''<br /> | ||
== References == | == References == | ||
<references/> | <references/> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
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