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5l80

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Revision as of 07:00, 5 August 2020

Structure of Exuperantia EXO-like and SAM-like domains

Structural highlights

5l80 is a 2 chain structure with sequence from Drome. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	5l7z
Gene:	exu, CG8994 (DROME)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[EXU_DROME] Ensures the proper localization of the mRNA of the bicoid gene to the anterior regions of the oocyte thus playing a fundamental role in the establishment of the polarity of the oocyte. May bind the bcd mRNA.

Publication Abstract from PubMed

Anterior patterning in Drosophila is mediated by the localization of bicoid (bcd) mRNA at the anterior pole of the oocyte. Exuperantia (Exu) is a putative exonuclease (EXO) associated with bcd and required for its localization. We present the crystal structure of Exu, which reveals a dimeric assembly with each monomer consisting of a 3'-5' EXO-like domain and a sterile alpha motif (SAM)-like domain. The catalytic site is degenerate and inactive. Instead, the EXO-like domain mediates dimerization and RNA binding. We show that Exu binds RNA directly in vitro, that the SAM-like domain is required for RNA binding activity and that Exu binds a structured element present in the bcd 3' untranslated region with high affinity. Through structure-guided mutagenesis, we show that Exu dimerization is essential for bcd localization. Our data demonstrate that Exu is a noncanonical RNA-binding protein with EXO-SAM-like domain architecture that interacts with its target RNA as a homodimer.

The bicoid mRNA localization factor Exuperantia is an RNA-binding pseudonuclease.,Lazzaretti D, Veith K, Kramer K, Basquin C, Urlaub H, Irion U, Bono F Nat Struct Mol Biol. 2016 Jul 4. doi: 10.1038/nsmb.3254. PMID:27376588^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lazzaretti D, Veith K, Kramer K, Basquin C, Urlaub H, Irion U, Bono F. The bicoid mRNA localization factor Exuperantia is an RNA-binding pseudonuclease. Nat Struct Mol Biol. 2016 Jul 4. doi: 10.1038/nsmb.3254. PMID:27376588 doi:http://dx.doi.org/10.1038/nsmb.3254

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5l80"

@@ Line 1: / Line 1: @@
 ==Structure of Exuperantia EXO-like and SAM-like domains==
-<StructureSection load='5l80' size='340' side='right' caption='[[5l80]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
+<StructureSection load='5l80' size='340' side='right'caption='[[5l80]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5l80]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L80 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5L80 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5l80]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L80 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5L80 FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5l7z|5l7z]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5l80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l80 OCA], [http://pdbe.org/5l80 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5l80 RCSB], [http://www.ebi.ac.uk/pdbsum/5l80 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5l80 ProSAT]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">exu, CG8994 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5l80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l80 OCA], [http://pdbe.org/5l80 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5l80 RCSB], [http://www.ebi.ac.uk/pdbsum/5l80 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5l80 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 22: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Drome]]
+[[Category: Large Structures]]
 [[Category: Bono, F]]
 [[Category: Lazzaretti, D]]

5l80

From Proteopedia

Revision as of 07:00, 5 August 2020

Structure of Exuperantia EXO-like and SAM-like domains

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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