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3c6e

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Revision as of 10:24, 12 August 2020

Crystal structure of the precursor membrane protein- envelope protein heterodimer from the dengue 2 virus at neutral pH

Structural highlights

3c6e is a 2 chain structure with sequence from Den26 and Dengue virus 2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Related:	3c5x
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[O09234_DEN26] Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).[SAAS:SAAS026470_004_099774]

Publication Abstract from PubMed

Many viruses go through a maturation step in the final stages of assembly before being transmitted to another host. The maturation process of flaviviruses is directed by the proteolytic cleavage of the precursor membrane protein (prM), turning inert virus into infectious particles. We have determined the 2.2 angstrom resolution crystal structure of a recombinant protein in which the dengue virus prM is linked to the envelope glycoprotein E. The structure represents the prM-E heterodimer and fits well into the cryo-electron microscopy density of immature virus at neutral pH. The pr peptide beta-barrel structure covers the fusion loop in E, preventing fusion with host cell membranes. The structure provides a basis for identifying the stages of its pH-directed conformational metamorphosis during maturation, ending with release of pr when budding from the host.

The flavivirus precursor membrane-envelope protein complex: structure and maturation.,Li L, Lok SM, Yu IM, Zhang Y, Kuhn RJ, Chen J, Rossmann MG Science. 2008 Mar 28;319(5871):1830-4. PMID:18369147^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Li L, Lok SM, Yu IM, Zhang Y, Kuhn RJ, Chen J, Rossmann MG. The flavivirus precursor membrane-envelope protein complex: structure and maturation. Science. 2008 Mar 28;319(5871):1830-4. PMID:18369147 doi:319/5871/1830

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3c6e"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the precursor membrane protein- envelope protein heterodimer from the dengue 2 virus at neutral pH==
-<StructureSection load='3c6e' size='340' side='right' caption='[[3c6e]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
+<StructureSection load='3c6e' size='340' side='right'caption='[[3c6e]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3c6e]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Den26 Den26] and [http://en.wikipedia.org/wiki/Dengue_virus_2 Dengue virus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C6E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3C6E FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3c6e]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Den26 Den26] and [http://en.wikipedia.org/wiki/Dengue_virus_2 Dengue virus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C6E OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3C6E FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3c5x|3c5x]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c6e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c6e OCA], [http://pdbe.org/3c6e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3c6e RCSB], [http://www.ebi.ac.uk/pdbsum/3c6e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3c6e ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3c6e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c6e OCA], [http://pdbe.org/3c6e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3c6e RCSB], [http://www.ebi.ac.uk/pdbsum/3c6e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3c6e ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 25: / Line 25: @@
 [[Category: Den26]]
 [[Category: Dengue virus 2]]
+[[Category: Large Structures]]
 [[Category: Li, L]]
 [[Category: Beta barrel]]

3c6e

From Proteopedia

Revision as of 10:24, 12 August 2020

Crystal structure of the precursor membrane protein- envelope protein heterodimer from the dengue 2 virus at neutral pH

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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