This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

5lsl

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 10:49, 12 August 2020

Crystal structure of yeast Hsh49p in complex with Cus1p binding domain.

Structural highlights

5lsl is a 8 chain structure with sequence from Atcc 18824. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	5lsb
Gene:	HSH49, YOR319W, O6142 (ATCC 18824), CUS1, YMR240C, YM9408.02C (ATCC 18824)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[HSH49_YEAST] Possible SF3b-like factor. [CUS1_YEAST] Essential splicing protein required for U2 snRNP binding to pre-mRNA during spliceosome assembly.

Publication Abstract from PubMed

Spliceosomal proteins Hsh49p and Cus1p are components of SF3b, which together with SF3a, Msl1p/Lea1p, Sm proteins and U2 snRNA, form U2 snRNP, which plays a crucial role in pre-mRNA splicing. Hsh49p, comprising two RRMs, forms a heterodimer with Cus1p. We determined the crystal structures of Saccharomyces cerevisiae full-length Hsh49p as well as its RRM1 in complex with a minimal binding region of Cus1p (residues 290-368). The structures show that the Cus1 fragment binds to the alpha-helical surface of Hsh49p RRM1, opposite the four-stranded beta-sheet, leaving the canonical RNA binding surface available to bind RNA. Hsh49p binds the 5' end region of U2 snRNA via RRM1. Its affinity is increased in complex with Cus1(290-368)p, partly because an extended RNA-binding surface forms across the protein-protein interface. The Hsh49p RRM1-Cus1(290-368)p structure fits well into cryo-EM density of the Bact spliceosome, corroborating the biological relevance of our crystal structure.

Crystal structure of U2 snRNP SF3b components: Hsh49p in complex with Cus1p binding domain.,van Roon AA, Oubridge C, Obayashi E, Sposito B, Newman AJ, Seraphin B, Nagai K RNA. 2017 Mar 27. pii: rna.059378.116. doi: 10.1261/rna.059378.116. PMID:28348170^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ van Roon AA, Oubridge C, Obayashi E, Sposito B, Newman AJ, Seraphin B, Nagai K. Crystal structure of U2 snRNP SF3b components: Hsh49p in complex with Cus1p binding domain. RNA. 2017 Mar 27. pii: rna.059378.116. doi: 10.1261/rna.059378.116. PMID:28348170 doi:http://dx.doi.org/10.1261/rna.059378.116

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5lsl"

@@ Line 1: / Line 1: @@
 ==Crystal structure of yeast Hsh49p in complex with Cus1p binding domain.==
-<StructureSection load='5lsl' size='340' side='right' caption='[[5lsl]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
+<StructureSection load='5lsl' size='340' side='right'caption='[[5lsl]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5lsl]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LSL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LSL FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5lsl]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LSL OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5LSL FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5lsb|5lsb]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lsl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lsl OCA], [http://pdbe.org/5lsl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lsl RCSB], [http://www.ebi.ac.uk/pdbsum/5lsl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lsl ProSAT]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HSH49, YOR319W, O6142 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), CUS1, YMR240C, YM9408.02C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5lsl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lsl OCA], [http://pdbe.org/5lsl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lsl RCSB], [http://www.ebi.ac.uk/pdbsum/5lsl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lsl ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 22: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Atcc 18824]]
+[[Category: Large Structures]]
 [[Category: Nagai, K]]
 [[Category: Obayashi, E]]

5lsl

From Proteopedia

Revision as of 10:49, 12 August 2020

Crystal structure of yeast Hsh49p in complex with Cus1p binding domain.

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox