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5mmt

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Revision as of 07:23, 19 August 2020

Inward open PepTSt from Streptococcus thermophilus crystallized in space group P3121

Structural highlights

5mmt is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Major facilitator superfamily (MFS) peptide transporters (typically referred to as PepT, POT or PTR transporters) mediate the uptake of di- and tripeptides, and so play an important dietary role in many organisms. In recent years, a better understanding of the molecular basis for this process has emerged, which is in large part due to a steep increase in structural information. Yet, the conformational transitions underlying the transport mechanism are still not fully understood, and additional data is therefore needed. Here we report in detail the detergent screening, crystallization, experimental MIRAS phasing, and refinement of the peptide transporter PepTSt from Streptococcus thermophilus. The space group is P3121, and the protein is crystallized in a monomeric inward facing form. The binding site is likely to be somewhat occluded, as the lobe encompassing transmembrane helices 10 and 11 is markedly bent towards the central pore of the protein, but the extent of this potential occlusion could not be determined due to disorder at the apex of the lobe. Based on structural comparisons with the seven previously determined P212121 and C2221 structures of inward facing PepTSt, the structural flexibility as well as the conformational changes mediating transition between the inward open and inward facing occluded states are discussed. In conclusion, this report improves our understanding of the structure and conformational cycle of PepTSt, and can furthermore serve as a case study, which may aid in supporting future structure determinations of additional MFS transporters or other integral membrane proteins.

Structure determination of a major facilitator peptide transporter: Inward facing PepTSt from Streptococcus thermophilus crystallized in space group P3121.,Quistgaard EM, Martinez Molledo M, Low C PLoS One. 2017 Mar 6;12(3):e0173126. doi: 10.1371/journal.pone.0173126., eCollection 2017. PMID:28264013^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Quistgaard EM, Martinez Molledo M, Low C. Structure determination of a major facilitator peptide transporter: Inward facing PepTSt from Streptococcus thermophilus crystallized in space group P3121. PLoS One. 2017 Mar 6;12(3):e0173126. doi: 10.1371/journal.pone.0173126., eCollection 2017. PMID:28264013 doi:http://dx.doi.org/10.1371/journal.pone.0173126

1 Structural highlights
2 Publication Abstract from PubMed
3 See Also
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5mmt"

@@ Line 1: / Line 1: @@
 ==Inward open PepTSt from Streptococcus thermophilus crystallized in space group P3121==
-<StructureSection load='5mmt' size='340' side='right' caption='[[5mmt]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
+<StructureSection load='5mmt' size='340' side='right'caption='[[5mmt]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5mmt]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MMT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MMT FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5mmt]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MMT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5MMT FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mmt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mmt OCA], [http://pdbe.org/5mmt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mmt RCSB], [http://www.ebi.ac.uk/pdbsum/5mmt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mmt ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5mmt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mmt OCA], [http://pdbe.org/5mmt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mmt RCSB], [http://www.ebi.ac.uk/pdbsum/5mmt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mmt ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 15: / Line 15: @@
 </div>
 <div class="pdbe-citations 5mmt" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Symporter 3D structures|Symporter 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Loew, C]]
 [[Category: Molledo, M M]]

5mmt

From Proteopedia

Revision as of 07:23, 19 August 2020

Inward open PepTSt from Streptococcus thermophilus crystallized in space group P3121

Structural highlights

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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