Journal:Acta Cryst D:S2059798320010906
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<b>Molecular Tour</b><br> | <b>Molecular Tour</b><br> | ||
| + | CYRI-B, also called FAM49B, is a well-conserved eukaryotic protein which interacts with the small GTPase Rac1 to regulate cellular actin assembly [1,2]. Through its interaction with Rac1, CYRI-B controls multiple critical cellular functions including T cell activation [1], chemotaxis and cell migration [2,3]. CYRI-B has also been described to reduce cellular entry of several bacterial pathogens [4] and has been suggested to play a role in various cancers [5, 6]. | ||
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| + | The structure of CYRI-B has been solved by X-ray crystallography at 2.4 Å resolution. It reveals an entirely α-helical protein organised into three distinct subdomains. The CYRI-B structure exhibits significant similarity with a conserved domain of CYFIP1, a key component of the WAVE regulatory complex (WRC) that interacts with Rac1 to promote actin polymerisation [7]. The study highlights residues crucial for Rac1 binding in both proteins. This suggests that CYRI-B interacts with Rac1 in the same manner as CYFIP1, regulating actin dependent processes by competition with the WRC for Rac1 binding. The structure provides a better understanding of the many roles played by CYRI-B, and also by other members of the CYRI protein family, in eukaryotic cells. | ||
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| + | References | ||
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| + | [1] Shang, W., Jiang, Y., Boettcher, M., Ding, K., Mollenauer, M., Liu, Z., Wen, X., Liu, C., Hao, P., Zhao, S., McManus, M. T., Wei, L., Weiss, A. & Wang, H. (2018). Proc. Natl. Acad. Sci. U. S. A. 115, E4051–E4060. | ||
| + | [2] Fort, L., Batista, J. M., Thomason, P. A., Spence, H. J., Whitelaw, J. A., Tweedy, L., Greaves, J., Martin, K. J., Anderson, K. I., Brown, P., Lilla, S., Neilson, M. P., Tafelmeyer, P., Zanivan, S., Ismail, S., Bryant, D. M., Tomkinson, N. C. O., Chamberlain, L. H., Mastick, G. S., Insall, R. H. & Machesky, L. M. (2018). Nat. Cell Biol. 20, 1159–1171. | ||
| + | [3] Whitelaw, J. A., Lilla, S., Paul, N. R., Fort, L., Zanivan, S. & Machesky, L. M. (2019). Commun. Integr. Biol. 12, 112–118. | ||
| + | [4] Yuki, K. E., Marei, H., Fiskin, E., Eva, M. M., Gopal, A. A., Schwartzentruber, J. A., Majewski, J., Cellier, M., Mandl, J. N., Vidal, S. M., Malo, D. & Dikic, I. (2019). Nat. Microbiol. 4, 1516–1531. | ||
| + | [5] Chattaragada, M. S., Riganti, C., Sassoe, M., Principe, M., Santamorena, M. M., Roux, C., Curcio, C., Evangelista, A., Allavena, P., Salvia, R., Rusev, B., Scarpa, A., Cappello, P. & Novelli, F. (2018). Oncogene. 37, 697–709. | ||
| + | [6] Long, Y., Marian, T. A. & Wei, Z. (2019). Biochem. Biophys. Res. Commun. 513, 1027–1034. | ||
| + | [7] Chen, Z., Borek, D., Padrick, S. B., Gomez, T. S., Metlagel, Z., Ismail, A. M., Umetani, J., Billadeau, D. D., Otwinowski, Z. & Rosen, M. K. (2010). Nature. 468, 533–538. | ||
<b>References</b><br> | <b>References</b><br> | ||
Revision as of 12:36, 24 August 2020
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