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3eff

From Proteopedia

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{{Seed}}
 
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[[Image:3eff.png|left|200px]]
 
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==The Crystal Structure of Full-Length KcsA in its Closed Conformation==
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The line below this paragraph, containing "STRUCTURE_3eff", creates the "Structure Box" on the page.
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<StructureSection load='3eff' size='340' side='right'caption='[[3eff]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3eff]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Streptomyces_lividans Streptomyces lividans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EFF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3EFF FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3eff FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eff OCA], [http://pdbe.org/3eff PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3eff RCSB], [http://www.ebi.ac.uk/pdbsum/3eff PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3eff ProSAT]</span></td></tr>
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-->
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</table>
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{{STRUCTURE_3eff| PDB=3eff | SCENE= }}
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== Function ==
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[[http://www.uniprot.org/uniprot/KCSA_STRLI KCSA_STRLI]] Acts as a pH-gated potassium ion channel; changing the cytosolic pH from 7 to 4 opens the channel, although it is not clear if this is the physiological stimulus for channel opening. Monovalent cation preference is K(+) > Rb(+) > NH4(+) >> Na(+) > Li(+).<ref>PMID:7489706</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ef/3eff_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3eff ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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KcsA is a proton-activated, voltage-modulated K(+) channel that has served as the archetype pore domain in the Kv channel superfamily. Here, we have used synthetic antigen-binding fragments (Fabs) as crystallographic chaperones to determine the structure of full-length KcsA at 3.8 A, as well as that of its isolated C-terminal domain at 2.6 A. The structure of the full-length KcsA-Fab complex reveals a well-defined, 4-helix bundle that projects approximately 70 A toward the cytoplasm. This bundle promotes a approximately 15 degrees bending in the inner bundle gate, tightening its diameter and shifting the narrowest point 2 turns of helix below. Functional analysis of the full-length KcsA-Fab complex suggests that the C-terminal bundle remains whole during gating. We suggest that this structure likely represents the physiologically relevant closed conformation of KcsA.
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===The Crystal Structure of Full-Length KcsA in its Closed Conformation===
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Crystal structure of full-length KcsA in its closed conformation.,Uysal S, Vasquez V, Tereshko V, Esaki K, Fellouse FA, Sidhu SS, Koide S, Perozo E, Kossiakoff A Proc Natl Acad Sci U S A. 2009 Apr 3. PMID:19346472<ref>PMID:19346472</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3eff" style="background-color:#fffaf0;"></div>
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<!--
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19346472}}, adds the Publication Abstract to the page
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*[[Antibody 3D structures|Antibody 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19346472 is the PubMed ID number.
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*[[Potassium channel 3D structures|Potassium channel 3D structures]]
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*[[3D structures of non-human antibody|3D structures of non-human antibody]]
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{{ABSTRACT_PUBMED_19346472}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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3EFF is a 8 chains structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Streptomyces_lividans Streptomyces lividans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EFF OCA].
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</StructureSection>
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[[Category: Large Structures]]
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==Reference==
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<ref group="xtra">PMID:19346472</ref><references group="xtra"/>
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[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Streptomyces lividans]]
[[Category: Streptomyces lividans]]
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[[Category: Esaki, K.]]
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[[Category: Esaki, K]]
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[[Category: Fellouse,F A.]]
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[[Category: Fellouse, F A]]
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[[Category: Koide, S.]]
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[[Category: Koide, S]]
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[[Category: Kossiakoff, A.]]
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[[Category: Kossiakoff, A]]
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[[Category: Perozo, E.]]
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[[Category: Perozo, E]]
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[[Category: Sidhu, S S.]]
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[[Category: Sidhu, S S]]
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[[Category: Tereshko, T.]]
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[[Category: Tereshko, T]]
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[[Category: Uysal, S.]]
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[[Category: Uysal, S]]
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[[Category: Vasquez, V.]]
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[[Category: Vasquez, V]]
[[Category: Bulge helix]]
[[Category: Bulge helix]]
[[Category: Cell membrane]]
[[Category: Cell membrane]]
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[[Category: Transport]]
[[Category: Transport]]
[[Category: Voltage-gated channel]]
[[Category: Voltage-gated channel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 6 10:57:59 2009''
 

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The Crystal Structure of Full-Length KcsA in its Closed Conformation

PDB ID 3eff

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