6s9u
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6s9u is ON HOLD Authors: Capra, N., Franceus, J., Desmet, T., Thunnissen, A.M.W.H. Description: Crystal structure of sucrose 6-phosphate phosphoryl...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of sucrose 6F-phosphate phosphorylase from Ilumatobacter coccineus== | |
| + | <StructureSection load='6s9u' size='340' side='right'caption='[[6s9u]], [[Resolution|resolution]] 2.05Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6s9u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Acidimicrobidae_bacterium_ym16-304 Acidimicrobidae bacterium ym16-304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S9U OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6S9U FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=B3P:2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>B3P</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">YM304_32550 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1313172 Acidimicrobidae bacterium YM16-304])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Transferase Transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.7 2.4.1.7] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6s9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s9u OCA], [http://pdbe.org/6s9u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6s9u RCSB], [http://www.ebi.ac.uk/pdbsum/6s9u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6s9u ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | In family GH13 of the carbohydrate-active enzyme database, subfamily 18 contains glycoside phosphorylases that act on alpha-sugars and glucosides. Because their phosphorolysis reactions are effectively reversible, these enzymes are of interest for the biocatalytic synthesis of various glycosidic compounds. Sucrose 6(F)-phosphate phosphorylases (SPPs) constitute one of the known substrate specificities. Here, we report the characterization of an SPP from Ilumatobacter coccineus with a far stricter specificity than the previously described promiscuous SPP from Thermoanaerobacterium thermosaccharolyticum. Crystal structures of both SPPs were determined to provide insight into their similarities and differences. The residues responsible for binding the fructose 6-phosphate group in subsite +1 were found to differ considerably between the two enzymes. Furthermore, several variants that introduce a higher degree of substrate promiscuity in the strict SPP from I. coccineus were designed. These results contribute to an expanded structural knowledge of enzymes in subfamily GH13_18 and facilitate their rational engineering. | ||
| - | + | Structural Comparison of a Promiscuous and a Highly Specific Sucrose 6(F)-Phosphate Phosphorylase.,Franceus J, Capra N, Desmet T, Thunnissen AWH Int J Mol Sci. 2019 Aug 11;20(16). pii: ijms20163906. doi: 10.3390/ijms20163906. PMID:31405215<ref>PMID:31405215</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6s9u" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Acidimicrobidae bacterium ym16-304]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Transferase]] | ||
[[Category: Capra, N]] | [[Category: Capra, N]] | ||
[[Category: Desmet, T]] | [[Category: Desmet, T]] | ||
| + | [[Category: Franceus, J]] | ||
| + | [[Category: Thunnissen, A M.W H]] | ||
| + | [[Category: Glycoside hydrolase family gh13-18]] | ||
| + | [[Category: Glycoside phosphorylase]] | ||
| + | [[Category: Sucrose 6-phosphate]] | ||
Current revision
Crystal structure of sucrose 6F-phosphate phosphorylase from Ilumatobacter coccineus
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