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6s9v

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Revision as of 07:34, 30 September 2020

Crystal structure of sucrose 6F-phosphate phosphorylase from Thermoanaerobacter thermosaccharolyticum

Structural highlights

6s9v is a 2 chain structure with sequence from Clostridium thermosaccharolyticum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , ,
Gene:	spp, Tthe_1921 (Clostridium thermosaccharolyticum)
Activity:	Sucrose 6(F)-phosphate phosphorylase, with EC number 2.4.1.329
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SUCPP_THETC] Catalyzes the reversible phosphorolysis of sucrose 6(F)-phosphate into alpha-D-glucose 1-phosphate (Glc1P) and D-fructose 6-phosphate. May be involved in a new pathway for the degradation of sucrose, which could become phosphorylated on its fructose moiety during uptake via a PTS system. To a lesser extent, can also reversibly act on sucrose in vitro (PubMed:24599311). Is also able to catalyze transglycosylation reactions in vitro (PubMed:26074151).^[1] ^[2]

Publication Abstract from PubMed

In family GH13 of the carbohydrate-active enzyme database, subfamily 18 contains glycoside phosphorylases that act on alpha-sugars and glucosides. Because their phosphorolysis reactions are effectively reversible, these enzymes are of interest for the biocatalytic synthesis of various glycosidic compounds. Sucrose 6(F)-phosphate phosphorylases (SPPs) constitute one of the known substrate specificities. Here, we report the characterization of an SPP from Ilumatobacter coccineus with a far stricter specificity than the previously described promiscuous SPP from Thermoanaerobacterium thermosaccharolyticum. Crystal structures of both SPPs were determined to provide insight into their similarities and differences. The residues responsible for binding the fructose 6-phosphate group in subsite +1 were found to differ considerably between the two enzymes. Furthermore, several variants that introduce a higher degree of substrate promiscuity in the strict SPP from I. coccineus were designed. These results contribute to an expanded structural knowledge of enzymes in subfamily GH13_18 and facilitate their rational engineering.

Structural Comparison of a Promiscuous and a Highly Specific Sucrose 6(F)-Phosphate Phosphorylase.,Franceus J, Capra N, Desmet T, Thunnissen AWH Int J Mol Sci. 2019 Aug 11;20(16). pii: ijms20163906. doi: 10.3390/ijms20163906. PMID:31405215^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Verhaeghe T, Aerts D, Diricks M, Soetaert W, Desmet T. The quest for a thermostable sucrose phosphorylase reveals sucrose 6'-phosphate phosphorylase as a novel specificity. Appl Microbiol Biotechnol. 2014 Aug;98(16):7027-37. doi:, 10.1007/s00253-014-5621-y. Epub 2014 Mar 6. PMID:24599311 doi:http://dx.doi.org/10.1007/s00253-014-5621-y
↑ Dirks-Hofmeister ME, Verhaeghe T, De Winter K, Desmet T. Creating Space for Large Acceptors: Rational Biocatalyst Design for Resveratrol Glycosylation in an Aqueous System. Angew Chem Int Ed Engl. 2015 Aug 3;54(32):9289-92. doi: 10.1002/anie.201503605., Epub 2015 Jun 12. PMID:26074151 doi:http://dx.doi.org/10.1002/anie.201503605
↑ Franceus J, Capra N, Desmet T, Thunnissen AWH. Structural Comparison of a Promiscuous and a Highly Specific Sucrose 6(F)-Phosphate Phosphorylase. Int J Mol Sci. 2019 Aug 11;20(16). pii: ijms20163906. doi: 10.3390/ijms20163906. PMID:31405215 doi:http://dx.doi.org/10.3390/ijms20163906

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6s9v"

@@ Line 3: / Line 3: @@
 <StructureSection load='6s9v' size='340' side='right'caption='[[6s9v]], [[Resolution|resolution]] 1.83&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6s9v]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S9V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6S9V FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6s9v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_thermosaccharolyticum Clostridium thermosaccharolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S9V OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6S9V FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">spp, Tthe_1921 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=580327 Clostridium thermosaccharolyticum])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Sucrose_6(F)-phosphate_phosphorylase Sucrose 6(F)-phosphate phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.329 2.4.1.329] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6s9v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s9v OCA], [http://pdbe.org/6s9v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6s9v RCSB], [http://www.ebi.ac.uk/pdbsum/6s9v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6s9v ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6s9v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s9v OCA], [http://pdbe.org/6s9v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6s9v RCSB], [http://www.ebi.ac.uk/pdbsum/6s9v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6s9v ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 23: / Line 24: @@
 __TOC__
 </StructureSection>
+[[Category: Clostridium thermosaccharolyticum]]
 [[Category: Large Structures]]
 [[Category: Capra, N]]

6s9v

From Proteopedia

Revision as of 07:34, 30 September 2020

Crystal structure of sucrose 6F-phosphate phosphorylase from Thermoanaerobacter thermosaccharolyticum

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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