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Porphobilinogen synthase

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<StructureSection load='1h7n' size='450' side='right' caption='Yeast porphobilinogen synthase complex with laevulunic acid and Zn+2 ion (grey) (PDB entry [[1h7n]])' scene='52/526179/Cv/1'>
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<StructureSection load='' size='450' side='right' caption='Yeast porphobilinogen synthase complex with laevulunic acid and Zn+2 ion (grey) (PDB entry [[1h7n]])' scene='52/526179/Cv/1'>
== Function ==
== Function ==
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'''Porphobilinogen synthase''' (PBS) catalyzes the formation of porphobilinogen (PBG) from 2 molecules of aminolaevulinic acid (ALA). PBS participates in porphyrin biosynthesis<ref>PMID:15381398</ref>. Levulinic acid (LA) is an inhibitor of PBS. Porphyrin is the precursor of hemes, chlorophyll and vitamin B12.
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'''Porphobilinogen synthase''' or '''δ-aminolaevulinic acid dehydratase''' (PBS) catalyzes the formation of porphobilinogen (PBG) from 2 molecules of aminolaevulinic acid (ALA). PBS participates in porphyrin biosynthesis<ref>PMID:15381398</ref>. Levulinic acid (LA) is an inhibitor of PBS. Porphyrin is the precursor of hemes, chlorophyll and vitamin B12.
== Disease ==
== Disease ==
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== Structural highlights ==
== Structural highlights ==
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The biological assembly of Yeast porphobilinogen synthase is <scene name='52/526179/Cv/2'>homooctamer</scene>. The <scene name='52/526179/Cv/3'>active site of PBS contains the substrate laevulinic acid and Zn+2 ion</scene><ref>PMID:11545591</ref>. Water molecules shown as red spheres.
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The biological assembly of Yeast porphobilinogen synthase is <scene name='52/526179/Cv/4'>homooctamer</scene>. The <scene name='52/526179/Cv/5'>active site of PBS contains the substrate laevulinic acid and Zn+2 ion</scene><ref>PMID:11545591</ref>. Water molecules are shown as red spheres.
</StructureSection>
</StructureSection>
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*Porphobilinogen synthase
*Porphobilinogen synthase
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**[[5hms]], [[5hnr]] – hPBS + Zn – human<br />
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**[[1pv8]] – hPBS (mutant)<br />
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**[[2z0i]], [[2z1b]] - PBS - mouse<br />
**[[1aw5]] – yPBS (mutant) + Zn – yeast<br />
**[[1aw5]] – yPBS (mutant) + Zn – yeast<br />
**[[1qml]] - yPBS + Hg<br />
**[[1qml]] - yPBS + Hg<br />
**[[1qnv]] - yPBS + Pb<br />
**[[1qnv]] - yPBS + Pb<br />
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**[[1pv8]] – hPBS (mutant) – human<br />
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**[[1w54]], [[1w56]], [[1w5m]], [[1w5n]], [[1w5o]], [[1w5p]], [[1w5q]] - PaPBS (mutant) + Mg + Zn – ''Pseudomonas aeruginosa''<br />
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**[[2z0i]], [[2z1b]] - PBS - mouse<br />
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**[[5lzl]] - PBS + Zn – ''Pyrobaculum calidifontis''<br />
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**[[1w54]], [[1w56]], [[1w5m]], [[1w5n]], [[1w5o]], [[1w5p]], [[1w5q]] - PaPBS (mutant) + Mg + Zn – ''Pseudomonas aeruginosa''
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*Porphobilinogen synthase complex
*Porphobilinogen synthase complex
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**[[1b4k]] - PaPBS + LA + Zn<br />
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**[[1e51]] - hPBS + PBG + Zn<br />
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**[[1b4e]] - EcPBS + LA + Zn – ''Escherichia coli''<br />
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**[[1ylv]], [[1h7n]] - yPBS + LA + Zn<br />
**[[1ylv]], [[1h7n]] - yPBS + LA + Zn<br />
**[[1w31]] - yPBS + LA derivative + Zn<br />
**[[1w31]] - yPBS + LA derivative + Zn<br />
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**[[1h7r]], [[1h7p]], [[1eb3]], [[1gjp]] - yPBS + inhibitor + Zn<br />
**[[1h7r]], [[1h7p]], [[1eb3]], [[1gjp]] - yPBS + inhibitor + Zn<br />
**[[1ohl]] - yPBS + intermediate + Zn<br />
**[[1ohl]] - yPBS + intermediate + Zn<br />
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**[[1b4e]] - EcPBS + LA + Zn – ''Escherichia coli''<br />
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**[[5mhb]] - EcPBS + PBG + Zn<br />
**[[1i8j]], [[1l6s]], [[1l6y]] - EcPBS + inhibitor + Mg + Zn<br />
**[[1i8j]], [[1l6s]], [[1l6y]] - EcPBS + inhibitor + Mg + Zn<br />
**[[2c1h]] - PBS + inhibitor + Mg – ''Chlorobium vibrioforme'' <br />
**[[2c1h]] - PBS + inhibitor + Mg – ''Chlorobium vibrioforme'' <br />
**[[2c13]], [[2c14]] - PaPBS (mutant) + inhibitor + Mg<br />
**[[2c13]], [[2c14]] - PaPBS (mutant) + inhibitor + Mg<br />
**[[2c15]], [[2c16]], [[2c18]], [[2c19]] - PaPBS + inhibitor + Mg<br />
**[[2c15]], [[2c16]], [[2c18]], [[2c19]] - PaPBS + inhibitor + Mg<br />
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**[[1e51]] - hPBS + PBG + Zn<br />
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**[[1b4k]] - PaPBS + LA + Zn<br />
**[[1gzg]] - PaPBS + LA derivative + Mg<br />
**[[1gzg]] - PaPBS + LA derivative + Mg<br />
**[[2woq]] - PaPBS + antibiotic + Mg<br />
**[[2woq]] - PaPBS + antibiotic + Mg<br />
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**[[1w1z]] - PBS + LA + Mg – ''Prosthecochloris vibrioformis''
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**[[1w1z]] - PBS + LA + Mg – ''Prosthecochloris vibrioformis''<br />
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**[[3obk]] - PBS residues 303-658 + Mg + PBG – ''Toxoplasma gondii''<br />
}}
}}
== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Yeast porphobilinogen synthase complex with laevulunic acid and Zn+2 ion (grey) (PDB entry 1h7n)

Drag the structure with the mouse to rotate

3D structures of porphobilinogen synthase

Updated on 06-October-2020

References

  1. ↑ Jaffe EK. The porphobilinogen synthase catalyzed reaction mechanism. Bioorg Chem. 2004 Oct;32(5):316-25. PMID:15381398 doi:http://dx.doi.org/10.1016/j.bioorg.2004.05.010
  2. ↑ Doss M, Muller WA. Acute lead poisoning in inherited porphobilinogen synthase (delta-aminolevulinic acid dehydrase) deficiency. Blut. 1982 Aug;45(2):131-9. PMID:7104498
  3. ↑ Erskine PT, Newbold R, Brindley AA, Wood SP, Shoolingin-Jordan PM, Warren MJ, Cooper JB. The x-ray structure of yeast 5-aminolaevulinic acid dehydratase complexed with substrate and three inhibitors. J Mol Biol. 2001 Sep 7;312(1):133-41. PMID:11545591 doi:10.1006/jmbi.2001.4947

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Michal Harel, Alexander Berchansky, Joel L. Sussman

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