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5wtj

From Proteopedia

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Current revision

Crystal structure of an endonuclease

Structural highlights

5wtj is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:
Related:	5wtk
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[C2C2_LEPSD] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA, optimally 28 nucleotides in this system) (PubMed:27256883). When the appropriate sequences are cloned into the CRISPR array confers immunity to ssRNA(+) enterobacteria phage MS2 (PubMed:27256883). Cleaves linear ssRNA in a crRNA-dependent fashion, preferentially at U residues; has no activity on partially dsRNA, ssDNA or dsDNA (PubMed:27256883). RNA secondary structure surrounding the target influence the cleavage site and efficiency; unlike other CRISPR-Cas effectors C2c2 cleaves outside of the crRNA binding site (PubMed:27256883). In the presence of a viable RNA target others RNAs can also be degraded in vitro and probably also in vivo, suggesting this type of CRISPR-Cas might also prevent viral spread by inducing programmed cell death or dormancy (PubMed:27256883). This system has a 3' protospacer flanking site (PFS), it does not cleave when the 3' PFS is G (PFS is equivalent to PAM, the protospacer adjacent motif) (PubMed:27256883). Mutations of its active site residues results in an RNA-programmed RNA-binding protein (PubMed:27256883).^[1]

Publication Abstract from PubMed

C2c2, the effector of type VI CRISPR-Cas systems, has two RNase activities-one for cutting its RNA target and the other for processing the CRISPR RNA (crRNA). Here, we report the structures of Leptotrichia shahii C2c2 in its crRNA-free and crRNA-bound states. While C2c2 has a bilobed structure reminiscent of all other Class 2 effectors, it also exhibits different structural characteristics. It contains the REC lobe with a Helical-1 domain and the NUC lobe with two HEPN domains. The two RNase catalytic pockets responsible for cleaving pre-crRNA and target RNA are independently located on Helical-1 and HEPN domains, respectively. crRNA binding induces significant conformational changes that are likely to stabilize crRNA binding and facilitate target RNA recognition. These structures provide important insights into the molecular mechanism of dual RNase activities of C2c2 and establish a framework for its future engineering as a RNA editing tool.

Two Distant Catalytic Sites Are Responsible for C2c2 RNase Activities.,Liu L, Li X, Wang J, Wang M, Chen P, Yin M, Li J, Sheng G, Wang Y Cell. 2017 Jan 12;168(1-2):121-134.e12. doi: 10.1016/j.cell.2016.12.031. Epub, 2017 Jan 12. PMID:28086085^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Abudayyeh OO, Gootenberg JS, Konermann S, Joung J, Slaymaker IM, Cox DB, Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, Regev A, Lander ES, Koonin EV, Zhang F. C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector. Science. 2016 Aug 5;353(6299):aaf5573. doi: 10.1126/science.aaf5573. Epub 2016, Jun 2. PMID:27256883 doi:http://dx.doi.org/10.1126/science.aaf5573
↑ Liu L, Li X, Wang J, Wang M, Chen P, Yin M, Li J, Sheng G, Wang Y. Two Distant Catalytic Sites Are Responsible for C2c2 RNase Activities. Cell. 2017 Jan 12;168(1-2):121-134.e12. doi: 10.1016/j.cell.2016.12.031. Epub, 2017 Jan 12. PMID:28086085 doi:http://dx.doi.org/10.1016/j.cell.2016.12.031

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5wtj"

Categories: Large Structures | Liu, L | Wang, Y | Hydrolase

@@ Line 1: / Line 1: @@
 ==Crystal structure of an endonuclease==
-<StructureSection load='5wtj' size='340' side='right' caption='[[5wtj]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
+<StructureSection load='5wtj' size='340' side='right'caption='[[5wtj]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5wtj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WTJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WTJ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5wtj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WTJ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5WTJ FirstGlance]. <br>
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5wtk|5wtk]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wtj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wtj OCA], [http://pdbe.org/5wtj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wtj RCSB], [http://www.ebi.ac.uk/pdbsum/5wtj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wtj ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5wtj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wtj OCA], [http://pdbe.org/5wtj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wtj RCSB], [http://www.ebi.ac.uk/pdbsum/5wtj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wtj ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 23: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Liu, L]]
 [[Category: Wang, Y]]
 [[Category: Hydrolase]]

5wtj

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Current revision

Crystal structure of an endonuclease

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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