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5wxl

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Revision as of 07:19, 7 October 2020

Crystal structure of the Rrs1 and Rpf2 complex

Structural highlights

5wxl is a 4 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RPF2_YEAST] Required for biogenesis of the 60S ribosomal subunit.^[1] [RRS1_YEAST] Required for ribosome biogenesis.^[2]

Publication Abstract from PubMed

Eukaryotic small ribosomal subunits are first assembled into 90S pre-ribosomes. The complete 90S is a gigantic complex with a molecular mass of approximately five megadaltons. Here, we report the nearly complete architecture of Saccharomyces cerevisiae 90S determined from three cryo-electron microscopy single particle reconstructions at 4.5 to 8.7 angstrom resolution. The majority of the density maps were modeled and assigned to specific RNA and protein components. The nascent ribosome is assembled into isolated native-like substructures that are stabilized by abundant assembly factors. The 5' external transcribed spacer and U3 snoRNA nucleate a large subcomplex that scaffolds the nascent ribosome. U3 binds four sites of pre-rRNA, including a novel site on helix 27 but not the 3' side of the central pseudoknot, and crucially organizes the 90S structure. The 90S model provides significant insight into the principle of small subunit assembly and the function of assembly factors.

Molecular architecture of the 90S small subunit pre-ribosome.,Sun Q, Zhu X, Qi J, An W, Lan P, Tan D, Chen R, Wang B, Zheng S, Zhang C, Chen X, Zhang W, Chen J, Dong MQ, Ye K Elife. 2017 Feb 28;6. pii: e22086. doi: 10.7554/eLife.22086. PMID:28244370^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bogengruber E, Briza P, Doppler E, Wimmer H, Koller L, Fasiolo F, Senger B, Hegemann JH, Breitenbach M. Functional analysis in yeast of the Brix protein superfamily involved in the biogenesis of ribosomes. FEMS Yeast Res. 2003 Mar;3(1):35-43. PMID:12702244
↑ Tsuno A, Miyoshi K, Tsujii R, Miyakawa T, Mizuta K. RRS1, a conserved essential gene, encodes a novel regulatory protein required for ribosome biogenesis in Saccharomyces cerevisiae. Mol Cell Biol. 2000 Mar;20(6):2066-74. PMID:10688653
↑ Sun Q, Zhu X, Qi J, An W, Lan P, Tan D, Chen R, Wang B, Zheng S, Zhang C, Chen X, Zhang W, Chen J, Dong MQ, Ye K. Molecular architecture of the 90S small subunit pre-ribosome. Elife. 2017 Feb 28;6. pii: e22086. doi: 10.7554/eLife.22086. PMID:28244370 doi:http://dx.doi.org/10.7554/eLife.22086

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5wxl"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the Rrs1 and Rpf2 complex==
-<StructureSection load='5wxl' size='340' side='right' caption='[[5wxl]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+<StructureSection load='5wxl' size='340' side='right'caption='[[5wxl]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5wxl]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WXL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WXL FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5wxl]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WXL OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5WXL FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wxl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wxl OCA], [http://pdbe.org/5wxl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wxl RCSB], [http://www.ebi.ac.uk/pdbsum/5wxl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wxl ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5wxl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wxl OCA], [http://pdbe.org/5wxl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wxl RCSB], [http://www.ebi.ac.uk/pdbsum/5wxl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wxl ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 17: @@
 </div>
 <div class="pdbe-citations 5wxl" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Ribosome biogenesis protein|Ribosome biogenesis protein]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Ye, K]]
 [[Category: Zheng, S]]

5wxl

From Proteopedia

Revision as of 07:19, 7 October 2020

Crystal structure of the Rrs1 and Rpf2 complex

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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