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6yuo
From Proteopedia
(Difference between revisions)
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==Capsule O-acetyltransferase of Neisseria meningitidis serogroup A in complex with caged Gadolinium== | ==Capsule O-acetyltransferase of Neisseria meningitidis serogroup A in complex with caged Gadolinium== | ||
| - | <StructureSection load='6yuo' size='340' side='right'caption='[[6yuo]]' scene=''> | + | <StructureSection load='6yuo' size='340' side='right'caption='[[6yuo]], [[Resolution|resolution]] 2.20Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YUO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6YUO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6yuo]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Neimd Neimd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YUO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6YUO FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6yuo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yuo OCA], [http://pdbe.org/6yuo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6yuo RCSB], [http://www.ebi.ac.uk/pdbsum/6yuo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6yuo ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GD3:GADOLINIUM+ION'>GD3</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sacC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=65699 NEIMD])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6yuo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yuo OCA], [http://pdbe.org/6yuo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6yuo RCSB], [http://www.ebi.ac.uk/pdbsum/6yuo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6yuo ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | O-Acetylation of the capsular polysaccharide (CPS) of Neisseria meningitidis serogroup A (NmA) is critical for the induction of functional immune responses, making this modification mandatory for CPS-based anti-NmA vaccines. Using comprehensive NMR studies, we demonstrate that O-acetylation stabilizes the labile anomeric phosphodiester-linkages of the NmA-CPS and occurs in position C3 and C4 of the N-acetylmannosamine units due to enzymatic transfer and non-enzymatic ester migration, respectively. To shed light on the enzymatic transfer mechanism, we solved the crystal structure of the capsule O-acetyltransferase CsaC in its apo and acceptor-bound form and of the CsaC-H228A mutant as trapped acetyl-enzyme adduct in complex with CoA. Together with the results of a comprehensive mutagenesis study, the reported structures explain the strict regioselectivity of CsaC and provide insight into the catalytic mechanism, which relies on an unexpected Gln-extension of a classical Ser-His-Asp triad, embedded in an alpha/beta-hydrolase fold. | ||
| + | |||
| + | Structural and mechanistic basis of capsule O-acetylation in Neisseria meningitidis serogroup A.,Fiebig T, Cramer JT, Bethe A, Baruch P, Curth U, Fuhring JI, Buettner FFR, Vogel U, Schubert M, Fedorov R, Muhlenhoff M Nat Commun. 2020 Sep 18;11(1):4723. doi: 10.1038/s41467-020-18464-y. PMID:32948778<ref>PMID:32948778</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6yuo" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Cramer | + | [[Category: Neimd]] |
| - | [[Category: Fedorov R]] | + | [[Category: Cramer, J T]] |
| - | [[Category: Fiebig T]] | + | [[Category: Fedorov, R]] |
| - | [[Category: Muehlenhoff M]] | + | [[Category: Fiebig, T]] |
| + | [[Category: Muehlenhoff, M]] | ||
| + | [[Category: A/b hydrolase fold]] | ||
| + | [[Category: Catalytic triad]] | ||
| + | [[Category: O-acetyltransferase]] | ||
| + | [[Category: Serine transferase]] | ||
| + | [[Category: Transferase]] | ||
Current revision
Capsule O-acetyltransferase of Neisseria meningitidis serogroup A in complex with caged Gadolinium
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