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3m3j
From Proteopedia
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| - | [[Image:3m3j.png|left|200px]] | ||
| - | + | ==A new crystal form of Lys48-linked diubiquitin== | |
| + | <StructureSection load='3m3j' size='340' side='right'caption='[[3m3j]], [[Resolution|resolution]] 1.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3m3j]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M3J OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3M3J FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3m3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m3j OCA], [http://pdbe.org/3m3j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3m3j RCSB], [http://www.ebi.ac.uk/pdbsum/3m3j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3m3j ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m3/3m3j_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3m3j ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Lys48-linked polyubiquitin chains are recognized by the proteasome as a tag for the degradation of the attached substrates. Here, a new crystal form of Lys48-linked diubiquitin (Ub(2)) was obtained and the crystal structure was refined to 1.6 A resolution. The structure reveals an ordered isopeptide bond in a trans configuration. All three molecules in the asymmetric unit were in the same closed conformation, in which the hydrophobic patches of both the distal and the proximal moieties interact with each other. Despite the different crystallization conditions and different crystal packing, the new crystal structure of Ub(2) is similar to the previously published structure of diubiquitin, but differences are observed in the conformation of the flexible isopeptide linkage. | ||
| - | + | A new crystal form of Lys48-linked diubiquitin.,Trempe JF, Brown NR, Noble ME, Endicott JA Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Sep 1;66(Pt, 9):994-8. Epub 2010 Aug 21. PMID:20823512<ref>PMID:20823512</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 3m3j" style="background-color:#fffaf0;"></div> | |
| - | + | ||
==See Also== | ==See Also== | ||
*[[Ubiquitin|Ubiquitin]] | *[[Ubiquitin|Ubiquitin]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
| - | [[Category: Brown, N R | + | [[Category: Large Structures]] |
| - | [[Category: Endicott, J A | + | [[Category: Brown, N R]] |
| - | [[Category: Noble, M E.M | + | [[Category: Endicott, J A]] |
| - | [[Category: Trempe, J F | + | [[Category: Noble, M E.M]] |
| + | [[Category: Trempe, J F]] | ||
[[Category: Beta grasp]] | [[Category: Beta grasp]] | ||
[[Category: Isopeptide bond]] | [[Category: Isopeptide bond]] | ||
Current revision
A new crystal form of Lys48-linked diubiquitin
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