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2vqi

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{{Seed}}
 
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[[Image:2vqi.png|left|200px]]
 
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==Structure of the P pilus usher (PapC) translocation pore==
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The line below this paragraph, containing "STRUCTURE_2vqi", creates the "Structure Box" on the page.
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<StructureSection load='2vqi' size='340' side='right'caption='[[2vqi]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2vqi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VQI OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2VQI FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene></td></tr>
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-->
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_2vqi| PDB=2vqi | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2vqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vqi OCA], [http://pdbe.org/2vqi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vqi RCSB], [http://www.ebi.ac.uk/pdbsum/2vqi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vqi ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/PAPC_ECOLX PAPC_ECOLX]] Involved in the export and assembly of pili subunits across the outer membrane. Forms a hexameric ring-shaped pore in the outer bacterial membrane. The 2 nanometer-diameter pore allows the passage of the thin tip fibrillum. As for the rod, it probably unwinds into linear fibers which would therefore be narrow enough to pass through the pore.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vq/2vqi_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vqi ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Gram-negative pathogens commonly exhibit adhesive pili on their surfaces that mediate specific attachment to the host. A major class of pili is assembled via the chaperone/usher pathway. Here, the structural basis for pilus fiber assembly and secretion performed by the outer membrane assembly platform--the usher--is revealed by the crystal structure of the translocation domain of the P pilus usher PapC and single particle cryo-electron microscopy imaging of the FimD usher bound to a translocating type 1 pilus assembly intermediate. These structures provide molecular snapshots of a twinned-pore translocation machinery in action. Unexpectedly, only one pore is used for secretion, while both usher protomers are used for chaperone-subunit complex recruitment. The translocating pore itself comprises 24 beta strands and is occluded by a folded plug domain, likely gated by a conformationally constrained beta-hairpin. These structures capture the secretion of a virulence factor across the outer membrane of gram-negative bacteria.
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===STRUCTURE OF THE P PILUS USHER (PAPC) TRANSLOCATION PORE===
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Fiber formation across the bacterial outer membrane by the chaperone/usher pathway.,Remaut H, Tang C, Henderson NS, Pinkner JS, Wang T, Hultgren SJ, Thanassi DG, Waksman G, Li H Cell. 2008 May 16;133(4):640-52. PMID:18485872<ref>PMID:18485872</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<!--
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_18485872}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2vqi" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 18485872 is the PubMed ID number.
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== References ==
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-->
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<references/>
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{{ABSTRACT_PUBMED_18485872}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Bacillus coli migula 1895]]
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2VQI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VQI OCA].
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[[Category: Large Structures]]
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[[Category: Henderson, N S]]
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==Reference==
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[[Category: Hultgren, S J]]
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Fiber formation across the bacterial outer membrane by the chaperone/usher pathway., Remaut H, Tang C, Henderson NS, Pinkner JS, Wang T, Hultgren SJ, Thanassi DG, Waksman G, Li H, Cell. 2008 May 16;133(4):640-52. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18485872 18485872]
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[[Category: Li, H]]
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[[Category: Escherichia coli]]
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[[Category: Pinkner, J S]]
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[[Category: Single protein]]
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[[Category: Remaut, H]]
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[[Category: Henderson, N S.]]
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[[Category: Tang, C]]
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[[Category: Hultgren, S J.]]
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[[Category: Thanassi, D G]]
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[[Category: Li, H.]]
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[[Category: Waksman, G]]
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[[Category: Pinkner, J S.]]
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[[Category: Wang, T]]
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[[Category: Remaut, H.]]
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[[Category: Tang, C.]]
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[[Category: Thanassi, D G.]]
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[[Category: Waksman, G.]]
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[[Category: Wang, T.]]
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[[Category: Fimbrium]]
[[Category: Fimbrium]]
[[Category: Membrane]]
[[Category: Membrane]]
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[[Category: Transport]]
[[Category: Transport]]
[[Category: Usher]]
[[Category: Usher]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:20:53 2008''
 

Current revision

Structure of the P pilus usher (PapC) translocation pore

PDB ID 2vqi

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