This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

5zho

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 21:19, 28 October 2020

Human group C rotavirus VP8*s recognize type A histo-blood group antigens as ligands

Structural highlights

5zho is a 1 chain structure with sequence from Human rotavirus (group c / strain bristol). Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[VP4_ROTHC] Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors (By similarity). Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm (By similarity). VP8* forms the head of the spikes. It is the viral hemagglutinin and an important target of neutralizing antibodies (By similarity).

Publication Abstract from PubMed

Group/species C rotaviruses (RVCs) have been identified as important pathogens of acute gastroenteritis (AGE) in children, family-based outbreaks, as well as animal infections. However, little is known regarding their host-specific interaction, infection, and pathogenesis. In this study, we performed serial studies to characterize the function and structural features of a human G4P[lsqb]2[rsqb] RVC VP8* that is responsible for host receptor interaction. Glycan microarrays demonstrated that the human RVC VP8* recognizes type A histo-blood antigens (HBGAs), which was confirmed by synthetic glycan-/saliva-based binding assays, and hemagglutination of red blood cells, establishing a paradigm of RVC VP8*-glycan interactions. Furthermore, high resolution crystal structure of the human RVC VP8* was solved, showing a typical galectin-like structure consisting of two beta-sheets but with significant differences to cogent proteins of RVAs. The VP8* in complex with a type A trisaccharide displays a novel ligand binding site that consists of a particular set of amino acid (aa) residues of the C-D, G-H, and K-L loops. RVC VP8* interacts with type A HBGAs through a unique mechanism compared with RVAs. Our findings shed light on the host-virus interaction and co-evolution of RVCs and will facilitate the development of specific antivirals and vaccines.IMPORTANCE Group/species C rotaviruses (RVCs), members of Reoviridae family, infect both humans and animals, but our knowledge about the host factors that control host susceptibility and specificity is rudimentary. In this work, we characterized the glycan binding specificity and structural basis of a human RVC that recognizes type A HBGAs. We found that human RVC VP8*, the RV host ligand binding domain that shares only approximately 15% homology to those of RVAs, recognizes type A HBGA at an as-yet-unknown glycan binding site through a distinct mechanism compared with RVAs. Our new advancements provide insights into RVC-cell attachment, the critical step of virus infection, which will in turn help the development of control and prevention strategies against RVs.

Human group C rotavirus VP8*s recognize type A histo-blood group antigens as ligands.,Sun X, Wang L, Qi J, Li D, Wang M, Cong X, Peng R, Chai W, Zhang Q, Wang H, Wen H, Gao GF, Tan M, Duan Z J Virol. 2018 Mar 28. pii: JVI.00442-18. doi: 10.1128/JVI.00442-18. PMID:29593033^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sun X, Wang L, Qi J, Li D, Wang M, Cong X, Peng R, Chai W, Zhang Q, Wang H, Wen H, Gao GF, Tan M, Duan Z. Human group C rotavirus VP8*s recognize type A histo-blood group antigens as ligands. J Virol. 2018 Mar 28. pii: JVI.00442-18. doi: 10.1128/JVI.00442-18. PMID:29593033 doi:http://dx.doi.org/10.1128/JVI.00442-18

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5zho"

@@ Line 1: / Line 1: @@
 ==Human group C rotavirus VP8*s recognize type A histo-blood group antigens as ligands==
-<StructureSection load='5zho' size='340' side='right' caption='[[5zho]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
+<StructureSection load='5zho' size='340' side='right'caption='[[5zho]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5zho]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_rotavirus_(group_c_/_strain_bristol) Human rotavirus (group c / strain bristol)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZHO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZHO FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5zho]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_rotavirus_(group_c_/_strain_bristol) Human rotavirus (group c / strain bristol)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZHO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5ZHO FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zho FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zho OCA], [http://pdbe.org/5zho PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zho RCSB], [http://www.ebi.ac.uk/pdbsum/5zho PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zho ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5zho FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zho OCA], [http://pdbe.org/5zho PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zho RCSB], [http://www.ebi.ac.uk/pdbsum/5zho PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zho ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Duan, Z]]
 [[Category: Qi, J]]

5zho

From Proteopedia

Revision as of 21:19, 28 October 2020

Human group C rotavirus VP8*s recognize type A histo-blood group antigens as ligands

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox