Staphylococcal nuclease

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<StructureSection load='' size='350' side='right' scene='49/497241/Cv/1' caption='Stapphylococcal nuclease complex with inhibitor thymidine diphosphate and Ca+2 ion (green) [[1snc]]' pspeed='8'>
<StructureSection load='' size='350' side='right' scene='49/497241/Cv/1' caption='Stapphylococcal nuclease complex with inhibitor thymidine diphosphate and Ca+2 ion (green) [[1snc]]' pspeed='8'>
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'''Micrococcal nuclease''', or '''Staphylococcal nuclease''', is a monomeric Ca++ dependent enzyme of 149 amino acids that cleaves either DNA or RNA substrates. It is used for relatively non-specific cleavage of nucleic acids in molecular biology, and has been an important model system for the study of protein folding.
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'''Micrococcal nuclease''', or '''Staphylococcal nuclease''' or '''Nuclease A''' or '''thermonuclease''', is a monomeric Ca++ dependent enzyme of 149 amino acids that cleaves either DNA or RNA substrates. It is used for relatively non-specific cleavage of nucleic acids in molecular biology, and has been an important model system for the study of protein folding.
==Structure==
==Structure==
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*<scene name='49/497241/Cv/7'>Stapphylococcal nuclease interactons with inhibitor thymidine diphosphate (TDP) and Ca+2 ion</scene>. Water molecules are shown as red spheres.
*<scene name='49/497241/Cv/7'>Stapphylococcal nuclease interactons with inhibitor thymidine diphosphate (TDP) and Ca+2 ion</scene>. Water molecules are shown as red spheres.
*<scene name='49/497241/Cv/8'>Ca coordination site</scene>.
*<scene name='49/497241/Cv/8'>Ca coordination site</scene>.
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</StructureSection>
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==3D structures of Staphylococcal nuclease==
==3D structures of Staphylococcal nuclease==
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[[Staphylococcal nuclease 3D structures]]
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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</StructureSection>
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{{#tree:id=OrganizedByTopic|openlevels=0|
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*Staphylococcal nuclease
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**[[1sno]], [[1ey0]], [[1kaa]], [[1kab]], [[1kda]], [[1kdb]], [[1kdc]], [[1stg]], [[1sth]], [[1stn]], [[1syb]], [[1syc]], [[1syd]], [[1sye]], [[1syf]], [[1syg]] – SCN – ''Staphylococcus aureus''<br />
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**[[1tr5]], [[1u9r]], [[2pw5]], [[2pyk]], [[2pzw]] – SCN (mutant) – Room temperature
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**[[3sk4]], [[3sk5]], [[3sk6]], [[3sk8]], [[3sxh]], [[3t13]], [[3hej]], [[3hzx]], [[3nhh]], [[3itp]], [[3p75]], [[3lx0]], [[3meh]], [[3mvv]], [[3qoj]], [[3qol]], [[6amf]], [[3mhb]], [[3mxp]], [[3mz5]], [[3r3o]], [[3ruz]], [[3t16]], [[3tme]], [[3tp5]], [[3tp6]], [[3tp7]], [[3tp8]], [[3v2t]], [[3qb3]], [[3va5]], [[4df7]], [[4dfa]], [[4dgz]], [[4du9]], [[4e6i]], [[4eqn]], [[4eqo]], [[4eqp]], [[4evo]], [[4f7x]], [[4f8m]], [[2pw7]], [[2pzt]], [[2pzu]], [[2rdf]], [[2qdb]], [[2rbm]], [[3bdc]], [[3c1e]], [[3c1f]], [[3d4d]], [[3d6c]], [[3dmu]], [[3e5s]], [[3eji]], [[3ero]], [[3erq]], [[3d4w]], [[3d8g]], [[3dhq]], [[3h6m]], [[2f0e]], [[2f0f]], [[2f0g]], [[2f0h]], [[2f0i]], [[2f0j]], [[2f0k]], [[2f0l]], [[2f0m]], [[2f0n]], [[2f0o]], [[2f0p]], [[2f0q]], [[2f0s]], [[2f0t]], [[2f0u]], [[2f0v]], [[2f0w]], [[2oxp]], [[2oeo]], [[2of1]], [[2rks]], [[1snp]], [[1snq]], [[1joo]], [[1jor]], [[1tqo]], [[1tt2]], [[2exz]], [[2ey1]], [[2ey2]], [[2ey5]], [[2ey6]], [[2eyf]], [[2eyh]], [[2eyj]], [[2eyl]], [[2eym]], [[2eyo]], [[2eyp]], [[2f0d]], [[4ky7]], [[4ky6]], [[4ky5]], [[4kv6]], [[4kta]], [[4kjo]], [[4khv]], [[4kjn]], [[4kd4]], [[4kd3]], [[4k8j]], [[4k8i]], [[4k6d]], [[4k5x]], [[4k5w]], [[4k5v]], [[4k2l]], [[4k2k]], [[4k14]], [[4i65]], [[4j6h]], [[4id6]], [[4j1m]], [[4iz8]], [[4iun]], [[4ial]], [[4hth]], [[4hmj]], [[4hmi]], [[4h7b]], [[4g57]], [[1ena]], [[1ey4]], [[1ey5]], [[1ey6]], [[1ey7]], [[1ey8]], [[1ey9]], [[1eya]], [[1eyc]], [[1eyd]], [[1ez6]], [[1ez8]], [[1f2m]], [[1f2y]], [[1f2z]], [[1ihz]], [[1ii3]], [[1nuc]], [[1sta]], [[1stb]], [[1sty]], [[2snm]], [[3evq]], [[3shl]], [[3u9o]], [[4laa]], [[4me5]], [[4n9p]], [[4n9t]], [[4ndx]], [[4nkl]], [[4nmz]], [[4np5]], [[4odg]], [[1eqv]], [[1snm]], [[3np8]], [[3nqt]], [[3nxw]], [[3oso]], [[3owf]], [[3p1h]], [[3pmf]], [[4miu]], [[4ol7]], [[4pmb]], [[4pmc]], [[4pny]], [[4qb4]], [[4qf4]], [[4r8n]], [[4rkb]], [[4rkl]], [[4trd]], [[4zq3]], [[4zui]], [[4zuj]], [[6eek]] – SCN (mutant) <br />
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**[[2khs]] – SCN - NMR<BR />
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**[[2f3v]], [[1rkn]] – SCN residues 1-110 (mutant) – NMR<BR />
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**[[2pqe]], [[2m00]], [[2lkv]] – SCN residues 80-228 (mutant) – NMR<BR />
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**[[2f3w]] – SCN residues 1-110 – NMR<BR />
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**[[2sob]] - SCN residues 83-185 – NMR<br />
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*Staphylococcal nuclease complex
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**[[1jok]], [[1joq]], [[3s9w]], [[3sr1]] - SCN (mutant) + Ca + TBP<br />
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**[[1a2t]] – SCN (mutant) + mercaptoethanol<br />
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**[[1enc]], [[2enb]], [[2sns]], [[4wor]] – SCN + TDP<br />
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**[[1a2u]], [[1snd]], [[2nuc]], [[5c3w]], [[5c4h]], [[5c4z]], [[5deh]], [[5e1f]], [[5e3f]], [[5egt]], [[5i6w]], [[5i6y]], [[5i9p]], [[5iif]], [[5isr]], [[5j1z]], [[5j22]], [[5jav]], [[5kgu]], [[5kix]], [[5nuc]], [[5c3x]], [[5c5l]], [[5c5m]], [[5c6a]], [[5cc4]], [[5cgk]], [[5cr3]], [[5cv4]], [[5cv5]], [[5cv6]], [[5cv7]], [[5cv8]], [[5cv9]], [[5dau]], [[5ekk]], [[5ekl]], [[5f2d]], [[5hgt]], [[5hur]], [[5i9o]], [[5igb]], [[5igc]], [[5igd]], [[5ige]], [[5igf]], [[5igg]], [[5ioc]], [[5iod]], [[5job]], [[5k5p]], [[5kee]], [[5kru]], [[5kyi]], [[5kyl]], [[6amf]]. [[6eeg]], [[6b8r]] – SCN (mutant) + TDP<br />
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**[[1a3t]] – SCN (mutant) + TDP + fluoroethane thiol<br />
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**[[1a3u]], [[1a3v]] – SCN (mutant) + cyclic thiol<br />
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**[[1aex]], [[3nuc]], [[5nuc]] – SCN (mutant) + alkane thiol<br />
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**[[1nsn]] – SCN + antibody<br />
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}}
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==References==
==References==

Current revision

Stapphylococcal nuclease complex with inhibitor thymidine diphosphate and Ca+2 ion (green) 1snc

Drag the structure with the mouse to rotate

References

  1. ↑ Arnone AA, Bier CJ, Cotton FA, Day VW, Hazen EE Jr, Richardson DC, Richardson JS, Yonath A (1971) "A High Resolution Structure of an Inhibitor Complex of the Extracellular Nuclease of Staphylococcus aureus: I. Experimental Procedures and Chain Tracing," J. Biol. Chem. 246, 2303-2316
  2. ↑ Roche J, Caro JA, Norberto DR, Barthe P, Roumestand C, Schlessman JL, Garcia AE, Garcia-Moreno E B, Royer CA. Cavities determine the pressure unfolding of proteins. Proc Natl Acad Sci U S A. 2012 Apr 10. PMID:22496593 doi:10.1073/pnas.1200915109
  3. ↑ Chen J, Lu Z, Sakon J, Stites WE. Proteins with simplified hydrophobic cores compared to other packing mutants. Biophys Chem. 2004 Aug 1;110(3):239-48. PMID:15228960 doi:10.1016/j.bpc.2004.02.007
  4. ↑ Wynn R, Harkins PC, Richards FM, Fox RO. Mobile unnatural amino acid side chains in the core of staphylococcal nuclease. Protein Sci. 1996 Jun;5(6):1026-31. PMID:8762134 doi:http://dx.doi.org/10.1002/pro.5560050605
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