This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
6v0e
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==Lipophilic Envelope-spanning Tunnel B (LetB), Model 3== | ==Lipophilic Envelope-spanning Tunnel B (LetB), Model 3== | ||
| - | <StructureSection load='6v0e' size='340' side='right'caption='[[6v0e]]' scene=''> | + | <StructureSection load='6v0e' size='340' side='right'caption='[[6v0e]], [[Resolution|resolution]] 3.06Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V0E OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6V0E FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6v0e]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V0E OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6V0E FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6v0e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v0e OCA], [http://pdbe.org/6v0e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6v0e RCSB], [http://www.ebi.ac.uk/pdbsum/6v0e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6v0e ProSAT]</span></td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">yebT, b1834, JW1823 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6v0e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v0e OCA], [http://pdbe.org/6v0e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6v0e RCSB], [http://www.ebi.ac.uk/pdbsum/6v0e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6v0e ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/YEBT_ECOLI YEBT_ECOLI]] Component of a transport pathway that contributes to membrane integrity (PubMed:27795327). May directly span the intermembrane space, facilitating the transport of substrates across the periplasm (Probable).<ref>PMID:27795327</ref> <ref>PMID:28388411</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Gram-negative bacteria are surrounded by an outer membrane composed of phospholipids and lipopolysaccharide, which acts as a barrier and contributes to antibiotic resistance. The systems that mediate phospholipid trafficking across the periplasm, such as MCE (Mammalian Cell Entry) transporters, have not been well characterized. Our ~3.5 A cryo-EM structure of the E. coli MCE protein LetB reveals an ~0.6 megadalton complex that consists of seven stacked rings, with a central hydrophobic tunnel sufficiently long to span the periplasm. Lipids bind inside the tunnel, suggesting that it functions as a pathway for lipid transport. Cryo-EM structures in the open and closed states reveal a dynamic tunnel lining, with implications for gating or substrate translocation. Our results support a model in which LetB establishes a physical link between the two membranes and creates a hydrophobic pathway for the translocation of lipids across the periplasm. | ||
| + | |||
| + | LetB Structure Reveals a Tunnel for Lipid Transport across the Bacterial Envelope.,Isom GL, Coudray N, MacRae MR, McManus CT, Ekiert DC, Bhabha G Cell. 2020 Apr 30;181(3):653-664.e19. doi: 10.1016/j.cell.2020.03.030. PMID:32359438<ref>PMID:32359438</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6v0e" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Ecoli]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Bhabha G]] | + | [[Category: Bhabha, G]] |
| - | [[Category: Coudray N]] | + | [[Category: Coudray, N]] |
| - | [[Category: Ekiert | + | [[Category: Ekiert, D C]] |
| - | [[Category: Isom | + | [[Category: Isom, G L]] |
| - | [[Category: MacRae | + | [[Category: MacRae, M R]] |
| - | [[Category: McManus | + | [[Category: McManus, C T]] |
| + | [[Category: Bacterial cell envelope]] | ||
| + | [[Category: Conformational dynamic]] | ||
| + | [[Category: Letb]] | ||
| + | [[Category: Lipid transport]] | ||
| + | [[Category: Mce]] | ||
| + | [[Category: Yebt]] | ||
Revision as of 07:08, 25 November 2020
Lipophilic Envelope-spanning Tunnel B (LetB), Model 3
| |||||||||||
Categories: Ecoli | Large Structures | Bhabha, G | Coudray, N | Ekiert, D C | Isom, G L | MacRae, M R | McManus, C T | Bacterial cell envelope | Conformational dynamic | Letb | Lipid transport | Mce | Yebt
