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1ra4

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Revision as of 13:22, 16 December 2020

Crystal structure of the Methanococcus jannaschii L7Ae protein

Structural highlights

1ra4 is a 1 chain structure with sequence from Atcc 43067. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	RPL7AE, MJ1203 (ATCC 43067)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RL7A_METJA] Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, the RNA component of RNase P, box H/ACA, box C/D and box C'/D' sRNAs.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Archaeal ribosomal protein L7Ae is a multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal, box H/ACA, and box C/D sRNAs. The crystal structure of Methanocaldococcus jannaschii L7Ae has been determined to 1.45 A, and L7Ae's amino acid composition, evolutionary conservation, functional characteristics, and structural details have been analyzed. Comparison of the L7Ae structure to those of a number of related proteins with diverse functions has revealed significant structural homology which suggests that this protein fold is an ancient RNA-binding motif. Notably, the free M. jannaschii L7Ae structure is essentially identical to that with RNA bound, suggesting that RNA binding occurs through an induced-fit interaction. Circular dichroism experiments show that box C/D and C'/D' RNA motifs undergo conformational changes when magnesium or the L7Ae protein is added, corroborating the induced-fit model for L7Ae-box C/D RNA interactions.

The crystal structure of the Methanocaldococcus jannaschii multifunctional L7Ae RNA-binding protein reveals an induced-fit interaction with the box C/D RNAs.,Suryadi J, Tran EJ, Maxwell ES, Brown BA 2nd Biochemistry. 2005 Jul 19;44(28):9657-72. PMID:16008351^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Suryadi J, Tran EJ, Maxwell ES, Brown BA 2nd. The crystal structure of the Methanocaldococcus jannaschii multifunctional L7Ae RNA-binding protein reveals an induced-fit interaction with the box C/D RNAs. Biochemistry. 2005 Jul 19;44(28):9657-72. PMID:16008351 doi:http://dx.doi.org/10.1021/bi050568q

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ra4"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the Methanococcus jannaschii L7Ae protein==
-<StructureSection load='1ra4' size='340' side='right' caption='[[1ra4]], [[Resolution|resolution]] 1.86&Aring;' scene=''>
+<StructureSection load='1ra4' size='340' side='right'caption='[[1ra4]], [[Resolution|resolution]] 1.86&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1ra4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43067 Atcc 43067]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RA4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RA4 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1ra4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43067 Atcc 43067]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RA4 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1RA4 FirstGlance]. <br>
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RPL7AE, MJ1203 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2190 ATCC 43067])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ra4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ra4 OCA], [http://pdbe.org/1ra4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ra4 RCSB], [http://www.ebi.ac.uk/pdbsum/1ra4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ra4 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1ra4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ra4 OCA], [http://pdbe.org/1ra4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ra4 RCSB], [http://www.ebi.ac.uk/pdbsum/1ra4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ra4 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 37: / Line 37: @@
 </StructureSection>
 [[Category: Atcc 43067]]
+[[Category: Large Structures]]
 [[Category: Brown, B A]]
 [[Category: Maxwell, E S]]

1ra4

From Proteopedia

Revision as of 13:22, 16 December 2020

Crystal structure of the Methanococcus jannaschii L7Ae protein

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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