Journal:Acta Cryst F:S2053230X20016015
From Proteopedia
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<b>Molecular Tour</b><br> | <b>Molecular Tour</b><br> | ||
We were unable to express the wild-type kinase domain (1-346) of hematopoietic progenitor kinase 1 (HPK1, also called MAP4K1). We investigated the potential surface aggregation properties of a model of HPK1 and chose eight sites to investigate by yeast surface display. This led to a double mutant L221D F225E, which expressed in baculovirus-infected insect cells at multiple milligrams per liter of culture. Purification of this construct showed that it was truncated after residue 319, which led to a revised construct of HPK1(1-319) L221D F225E. This construct crystallized in the presence of inhibitors and led to a structure with two molecules/asymmetric unit. The activation loop on both molecules was domain-swapped, that is the activation loop of chain A bind to chain B and vice versa. We were able to use this construct to determine many structures of HPK1/inhibitor complexes in support a structure-based drug design effort. | We were unable to express the wild-type kinase domain (1-346) of hematopoietic progenitor kinase 1 (HPK1, also called MAP4K1). We investigated the potential surface aggregation properties of a model of HPK1 and chose eight sites to investigate by yeast surface display. This led to a double mutant L221D F225E, which expressed in baculovirus-infected insect cells at multiple milligrams per liter of culture. Purification of this construct showed that it was truncated after residue 319, which led to a revised construct of HPK1(1-319) L221D F225E. This construct crystallized in the presence of inhibitors and led to a structure with two molecules/asymmetric unit. The activation loop on both molecules was domain-swapped, that is the activation loop of chain A bind to chain B and vice versa. We were able to use this construct to determine many structures of HPK1/inhibitor complexes in support a structure-based drug design effort. | ||
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<b>References</b><br> | <b>References</b><br> | ||
Revision as of 13:22, 22 December 2020
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