6jx1
From Proteopedia
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6jx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jx1 OCA], [http://pdbe.org/6jx1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jx1 RCSB], [http://www.ebi.ac.uk/pdbsum/6jx1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jx1 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6jx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jx1 OCA], [http://pdbe.org/6jx1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jx1 RCSB], [http://www.ebi.ac.uk/pdbsum/6jx1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jx1 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Formate dehydrogenase (FDH) has been widely used for the regeneration of the reduced nicotinamide adenine dinucleotide (NADH). To utilize nicotinamide cytosine dinucleotide (NCD) as a non-natural redox cofactor, it remains challenging as NCDH, the reduced form of NCD, has to be efficiently regenerated. Here we demonstrate successful engineering of FDH for NCDH regeneration. Guided by the structural information of FDH from Pseudomonas sp. 101 (pseFDH) and the NAD-pseFDH complex, semi-rational strategies were applied to design mutant libraries and screen for NCD-linked activity. The most active mutant reached a cofactor preference switch from NAD to NCD by 3700-fold. Homology modeling analysis showed that these mutants had reduced cofactor binding pockets and dedicated hydrophobic interactions for NCD. Efficient regeneration of NCDH was implemented by powering an NCD-dependent D-lactate dehydrogenase for stoichiometric and stereospecific reduction of pyruvate to D-lactate at the expense of formate. | ||
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| + | Structure-Guided Design of Formate Dehydrogenase for Regeneration of a Non-Natural Redox Cofactor.,Guo X, Wang X, Liu Y, Li Q, Wang J, Liu W, Zhao ZK Chemistry. 2020 Dec 15;26(70):16611-16615. doi: 10.1002/chem.202003102. Epub 2020, Nov 16. PMID:32815230<ref>PMID:32815230</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6jx1" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[Formate dehydrogenase 3D structures|Formate dehydrogenase 3D structures]] | *[[Formate dehydrogenase 3D structures|Formate dehydrogenase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 06:53, 30 December 2020
Crystal structure of Formate dehydrogenase mutant V198I/C256I/P260S/E261P/S381N/S383F from Pseudomonas sp. 101
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