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Revision as of 07:55, 3 February 2021 (edit) Michal Harel (Talk | contribs) (New page: <StructureSection load='1st6' size='340' side='right' caption='Chicken full-length metavinculin, 1st6' scene='' > == Function == '''Ubiquitin-fold modifier''' (VCLs) are involved in ad...) ← Previous diff		Revision as of 10:23, 3 February 2021 (edit) (undo) Michal Harel (Talk | contribs) Next diff →
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	*Ubiquitin-fold modifier		*Ubiquitin-fold modifier
-	**[[1tr2]], [[6fuy]] – hVCL - human<br />	+	**[[5ia7]] – hUmf1 - human<br />
		+	**[[1wxs]] – hUmf1 - NMR<br />
		+	**[[5hkh]] – hUmf1 + peptide<br />
		+	**[[5iaa]] – hUmf1 + UBA5<br />
		+	**[[5l95]] – hUmf1 + UBA5 + AMP<br />
		+	**[[6h77]] – hUmf1 + UBA5 + ATP<br />
		+	**[[5xda]] – Umf1 + Umf1-specific protease - ''Caenorhabditis elegans''<br />
		+	**[[6izg]] – Umf1 - ''Trypanosoma brucei'' - NMR<br />
-	*Metavinculin; Domains - head 1-256; tail 959-1134	+	*Umf1-conjugating enzyme
		+	**[[2z6o]], [[2z6p]], [[3evx]] – hUfc1<br />
		+	**[[2k07]] – hUfc1 - NMR<br />
		+	*Ubiquitin-like modifier activating enzyme
		+
		+	**[[3h8v]], [[6h78]] – hUBA5 + ATP<br />
		+	**[[3guc]] – hUBA5 + AMPPNP<br />
	}}		}}
	==References==		==References==
	<references />		<references />
	[[Category:Topic Page]]		[[Category:Topic Page]]

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Revision as of 10:23, 3 February 2021

spinqualitylabelsall models Chicken full-length metavinculin, 1st6 Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image Contents 1 Function 2 Relevance 3 Disease 4 Structural highlights Function Ubiquitin-fold modifier (VCLs) are involved in adhesion by linking integrin molecules to the actin cytoskeleton. Its head domain (Vd1) can bind to talin or to alpha-actinin at their respective VCL Binding Sites (VBS)[1]. The protein raver1 RNA Recognition Motif (RRM) forms a complex with VCL or m-VCL. Metavinculin (m-VCL) is a splice version of VCL containing an extra ca. 70 amino acids in the C-terminal domain. Relevance Loss of VCL could be used as a prognostic factor for colorectal cancer se it promotes metastasis[2]. Disease Mutation in m-VCL can yield cardiomyopathic phenotype[3]. Structural highlights is achieved through a high affinity intramolecular interaction between tail (orange) and head (aqua) domains (1st6). Energetically, I997 is key to maintaining this autoinhibition.

3D Structures of Ubiquitin-fold modifier

Updated on 03-February-2021

References

1. ↑ Palovuori R, Eskelinen S. Role of vinculin in the maintenance of cell-cell contacts in kidney epithelial MDBK cells. Eur J Cell Biol. 2000 Dec;79(12):961-74. PMID:11152287 doi:http://dx.doi.org/10.1078/0171-9335-00120
2. ↑ Li T, Guo H, Song Y, Zhao X, Shi Y, Lu Y, Hu S, Nie Y, Fan D, Wu K. Loss of vinculin and membrane-bound beta-catenin promotes metastasis and predicts poor prognosis in colorectal cancer. Mol Cancer. 2014 Dec 11;13:263. doi: 10.1186/1476-4598-13-263. PMID:25496021 doi:http://dx.doi.org/10.1186/1476-4598-13-263
3. ↑ Vasile VC, Will ML, Ommen SR, Edwards WD, Olson TM, Ackerman MJ. Identification of a metavinculin missense mutation, R975W, associated with both hypertrophic and dilated cardiomyopathy. Mol Genet Metab. 2006 Feb;87(2):169-74. Epub 2005 Oct 19. PMID:16236538 doi:S1096-7192(05)00258-1