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2eho

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[[Image:2eho.jpg|left|200px]]<br /><applet load="2eho" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2eho, resolution 3.00&Aring;" />
 
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'''Crystal structure of human GINS complex'''<br />
 
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==Overview==
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==Crystal structure of human GINS complex==
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The GINS complex mediates the assembly of the MCM2-7 (minichromosome, maintenance) complex with proteins in a replisome progression complex. The, eukaryotic GINS complex is composed of Sld5, Psf1, Psf2, and Psf3, which, must be assembled for cell proliferation. We determined the crystal, structure of the human GINS complex: GINS forms an elliptical shape with a, small central channel. The structures of Sld5 and Psf2 resemble those of, Psf1 and Psf3, respectively. In addition, the N-terminal and C-terminal, domains of Sld5/Psf1 are permuted in Psf2/Psf3, which suggests that the, four proteins have evolved from a common ancestor. Using a structure-based, mutational analysis, we identified the functionally critical surface, regions of the GINS complex.
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<StructureSection load='2eho' size='340' side='right'caption='[[2eho]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2eho]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EHO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EHO FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Psf1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), PSF2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), Psf3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2eho FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eho OCA], [https://pdbe.org/2eho PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2eho RCSB], [https://www.ebi.ac.uk/pdbsum/2eho PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2eho ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/PSF3_HUMAN PSF3_HUMAN]] The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA.<ref>PMID:17417653</ref> [[https://www.uniprot.org/uniprot/SLD5_HUMAN SLD5_HUMAN]] The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS4 is important for GINS complex assembly. GINS complex seems to bind preferentially to single-stranded DNA.<ref>PMID:17417653</ref> [[https://www.uniprot.org/uniprot/PSF2_HUMAN PSF2_HUMAN]] The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA.<ref>PMID:17417653</ref> [[https://www.uniprot.org/uniprot/PSF1_HUMAN PSF1_HUMAN]] The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA. GINS1 is essential for function.<ref>PMID:17417653</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eh/2eho_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2eho ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The GINS complex mediates the assembly of the MCM2-7 (minichromosome maintenance) complex with proteins in a replisome progression complex. The eukaryotic GINS complex is composed of Sld5, Psf1, Psf2, and Psf3, which must be assembled for cell proliferation. We determined the crystal structure of the human GINS complex: GINS forms an elliptical shape with a small central channel. The structures of Sld5 and Psf2 resemble those of Psf1 and Psf3, respectively. In addition, the N-terminal and C-terminal domains of Sld5/Psf1 are permuted in Psf2/Psf3, which suggests that the four proteins have evolved from a common ancestor. Using a structure-based mutational analysis, we identified the functionally critical surface regions of the GINS complex.
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==About this Structure==
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Crystal structure of the human GINS complex.,Choi JM, Lim HS, Kim JJ, Song OK, Cho Y Genes Dev. 2007 Jun 1;21(11):1316-21. PMID:17545466<ref>PMID:17545466</ref>
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2EHO is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EHO OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of the human GINS complex., Choi JM, Lim HS, Kim JJ, Song OK, Cho Y, Genes Dev. 2007 Jun 1;21(11):1316-21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17545466 17545466]
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</div>
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[[Category: Homo sapiens]]
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<div class="pdbe-citations 2eho" style="background-color:#fffaf0;"></div>
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[[Category: Protein complex]]
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== References ==
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[[Category: Cho, Y.]]
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<references/>
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[[Category: Choi, J.M.]]
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__TOC__
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[[Category: Kim, J.J.]]
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</StructureSection>
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[[Category: Lim, H.S.]]
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[[Category: Human]]
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[[Category: Song, O.K.]]
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[[Category: Large Structures]]
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[[Category: SO4]]
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[[Category: Cho, Y]]
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[[Category: Choi, J M]]
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[[Category: Kim, J J]]
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[[Category: Lim, H S]]
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[[Category: Song, O K]]
[[Category: 4-helical bundle]]
[[Category: 4-helical bundle]]
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[[Category: hydrophobic interaction]]
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[[Category: Hydrophobic interaction]]
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[[Category: protein-protein complex]]
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[[Category: Protein-protein complex]]
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[[Category: Replication]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:48:36 2008''
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Current revision

Crystal structure of human GINS complex

PDB ID 2eho

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