1drf

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[[Image:1drf.gif|left|200px]]
[[Image:1drf.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1drf |SIZE=350|CAPTION= <scene name='initialview01'>1drf</scene>, resolution 2.0&Aring;
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The line below this paragraph, containing "STRUCTURE_1drf", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=FOL:FOLIC+ACID'>FOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1drf| PDB=1drf | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1drf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1drf OCA], [http://www.ebi.ac.uk/pdbsum/1drf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1drf RCSB]</span>
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}}
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'''CRYSTAL STRUCTURE OF HUMAN DIHYDROFOLATE REDUCTASE COMPLEXED WITH FOLATE'''
'''CRYSTAL STRUCTURE OF HUMAN DIHYDROFOLATE REDUCTASE COMPLEXED WITH FOLATE'''
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[[Category: Oefner, C.]]
[[Category: Oefner, C.]]
[[Category: Winkler, F K.]]
[[Category: Winkler, F K.]]
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[[Category: oxidoreductase (ch-nh(d)-nad or nadp(a))]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:11:20 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:47:12 2008''
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Revision as of 11:11, 2 May 2008

Image:1drf.gif

Template:STRUCTURE 1drf

CRYSTAL STRUCTURE OF HUMAN DIHYDROFOLATE REDUCTASE COMPLEXED WITH FOLATE


Overview

The crystal structure of recombinant human dihydrofolate reductase with folate bound in the active site has been determined and the structural model refined at 0.2-nm resolution. Preliminary studies of the binding of the inhibitors methotrexate and trimethoprim to the human apoenzyme have been performed at 0.35-nm resolution. The conformations of the chemically very similar ligands folate and methotrexate, one a substrate the other a potent inhibitor, differ substantially in that their pteridine rings are in inverse orientations relative to their p-aminobenzoyl-L-glutamate moieties. Methotrexate binding is similar to that previously observed in two bacterial enzymes but is quite different from that observed in the enzyme from a mouse lymphoma cell line [Stammers et al. (1987) FEBS Lett. 218, 178-184]. The geometry of the polypeptide chain around the folate binding site in the human enzyme is not consistent with conclusions previously drawn with regard to the species selectivity of the inhibitor trimethoprim [Matthews et al. (1985) J. Biol. Chem. 260, 392-399].

About this Structure

1DRF is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of human dihydrofolate reductase complexed with folate., Oefner C, D'Arcy A, Winkler FK, Eur J Biochem. 1988 Jun 1;174(2):377-85. PMID:3383852 Page seeded by OCA on Fri May 2 14:11:20 2008

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