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2fcj

From Proteopedia

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[[Image:2fcj.png|left|200px]]
 
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==Structure of small TOPRIM domain protein from Bacillus stearothermophilus.==
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The line below this paragraph, containing "STRUCTURE_2fcj", creates the "Structure Box" on the page.
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<StructureSection load='2fcj' size='340' side='right'caption='[[2fcj]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2fcj]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_12980 Atcc 12980]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FCJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FCJ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RBSTP2199 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1422 ATCC 12980])</td></tr>
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{{STRUCTURE_2fcj| PDB=2fcj | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fcj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fcj OCA], [https://pdbe.org/2fcj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fcj RCSB], [https://www.ebi.ac.uk/pdbsum/2fcj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fcj ProSAT], [https://www.topsan.org/Proteins/MCSG/2fcj TOPSAN]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fc/2fcj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fcj ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of the Midwest Center for Structural Genomics target APC35832, a 14.7-kDa cytosolic protein from Bacillus stearothermophilus, has been determined at 1.3 A resolution by the single anomalous diffraction method from a mercury soaked crystal. The APC35832 protein is a representative of large group of bacterial and archeal proteins entirely consisting of the Toprim (topoisomerase-primase) domain. This domain is found in the catalytic centers of many enzymes catalyzing phosphodiester bond formation or cleavage, but the function of small Toprim domain proteins remains unknown. Consistent with the sequence analysis, the APC35832 structure shows a conserved Toprim fold, with a central 4-stranded parallel beta-sheet surrounded by four alpha-helixes. Comparison of the APC35832 structure with its closest structural homolog, the catalytic core of bacteriophage T7 primase, revealed structural conservation of a metal binding site and isothermal titration calorimetry indicates that APC35832 binds Mg2+ with a sub-millimolar dissociation constant (K(d)). The APC35832-Mg2+ complex structure was determined at 1.65 A and reveals the role of conserved acidic residues in Mg2+ ion coordination. The structural similarities to other Toprim domain containing proteins and potential function and substrates of APC35832 are discussed in this article.
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===Structure of small TOPRIM domain protein from Bacillus stearothermophilus.===
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Crystal structure and putative function of small Toprim domain-containing protein from Bacillus stearothermophilus.,Rezacova P, Borek D, Moy SF, Joachimiak A, Otwinowski Z Proteins. 2008 Feb 1;70(2):311-9. PMID:17705269<ref>PMID:17705269</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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[[2fcj]] is a 3 chain structure of [[TOPRIM domain protein]] with sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FCJ OCA].
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<div class="pdbe-citations 2fcj" style="background-color:#fffaf0;"></div>
==See Also==
==See Also==
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*[[Acetylcholinesterase with OTMA|Acetylcholinesterase with OTMA]]
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*[[Central glycolytic gene regulator (CggR)|Central glycolytic gene regulator (CggR)]]
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*[[Acid beta-glucosidase with N-butyl-deoxynojirimycin|Acid beta-glucosidase with N-butyl-deoxynojirimycin]]
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*[[TOPRIM domain protein|TOPRIM domain protein]]
*[[TOPRIM domain protein|TOPRIM domain protein]]
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[[Category: Geobacillus stearothermophilus]]
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== References ==
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[[Category: Borek, D.]]
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<references/>
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[[Category: Chen, Y.]]
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__TOC__
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[[Category: Collart, F.]]
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</StructureSection>
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[[Category: Joachimiak, A.]]
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[[Category: Atcc 12980]]
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[[Category: MCSG, Midwest Center for Structural Genomics.]]
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[[Category: Large Structures]]
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[[Category: Otwinowski, Z.]]
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[[Category: Borek, D]]
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[[Category: Rezacova, P.]]
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[[Category: Chen, Y]]
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[[Category: Collart, F]]
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[[Category: Joachimiak, A]]
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[[Category: Structural genomic]]
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[[Category: Otwinowski, Z]]
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[[Category: Rezacova, P]]
[[Category: Mcsg]]
[[Category: Mcsg]]
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[[Category: Midwest center for structural genomic]]
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[[Category: PSI, Protein structure initiative]]
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[[Category: Protein structure initiative]]
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[[Category: Psi]]
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[[Category: Structural genomic]]
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[[Category: Toprim domain]]
[[Category: Toprim domain]]
[[Category: Unknown function]]
[[Category: Unknown function]]

Current revision

Structure of small TOPRIM domain protein from Bacillus stearothermophilus.

PDB ID 2fcj

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