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2fhd

From Proteopedia

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{{Seed}}
 
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[[Image:2fhd.png|left|200px]]
 
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==Crystal structure of Crb2 tandem tudor domains==
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The line below this paragraph, containing "STRUCTURE_2fhd", creates the "Structure Box" on the page.
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<StructureSection load='2fhd' size='340' side='right'caption='[[2fhd]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2fhd]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Cbs_356 Cbs 356]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FHD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FHD FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_2fhd| PDB=2fhd | SCENE= }}
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rhp9, crb2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 CBS 356])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fhd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fhd OCA], [https://pdbe.org/2fhd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fhd RCSB], [https://www.ebi.ac.uk/pdbsum/2fhd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fhd ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fh/2fhd_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fhd ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Histone lysine methylation has been linked to the recruitment of mammalian DNA repair factor 53BP1 and putative fission yeast homolog Crb2 to DNA double-strand breaks (DSBs), but how histone recognition is achieved has not been established. Here we demonstrate that this link occurs through direct binding of 53BP1 and Crb2 to histone H4. Using X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy, we show that, despite low amino acid sequence conservation, both 53BP1 and Crb2 contain tandem tudor domains that interact with histone H4 specifically dimethylated at Lys20 (H4-K20me2). The structure of 53BP1/H4-K20me2 complex uncovers a unique five-residue 53BP1 binding cage, remarkably conserved in the structure of Crb2, that best accommodates a dimethyllysine but excludes a trimethyllysine, thus explaining the methylation state-specific recognition of H4-K20. This study reveals an evolutionarily conserved molecular mechanism of targeting DNA repair proteins to DSBs by direct recognition of H4-K20me2.
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===Crystal structure of Crb2 tandem tudor domains===
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Structural basis for the methylation state-specific recognition of histone H4-K20 by 53BP1 and Crb2 in DNA repair.,Botuyan MV, Lee J, Ward IM, Kim JE, Thompson JR, Chen J, Mer G Cell. 2006 Dec 29;127(7):1361-73. PMID:17190600<ref>PMID:17190600</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<!--
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_17190600}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2fhd" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17190600 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17190600}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Cbs 356]]
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2FHD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FHD OCA].
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[[Category: Large Structures]]
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[[Category: Botuyan, M V]]
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==Reference==
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[[Category: Lee, J]]
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Structural basis for the methylation state-specific recognition of histone H4-K20 by 53BP1 and Crb2 in DNA repair., Botuyan MV, Lee J, Ward IM, Kim JE, Thompson JR, Chen J, Mer G, Cell. 2006 Dec 29;127(7):1361-73. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17190600 17190600]
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[[Category: Mer, G]]
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[[Category: Schizosaccharomyces pombe]]
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[[Category: Thompson, J R]]
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[[Category: Single protein]]
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[[Category: Cell cycle]]
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[[Category: Botuyan, M V.]]
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[[Category: Lee, J.]]
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[[Category: Mer, G.]]
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[[Category: Thompson, J R.]]
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[[Category: Tamdem tudor domain]]
[[Category: Tamdem tudor domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:00:35 2008''
 

Current revision

Crystal structure of Crb2 tandem tudor domains

PDB ID 2fhd

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