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2fs2

From Proteopedia

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[[Image:2fs2.gif|left|200px]]<br /><applet load="2fs2" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2fs2, resolution 2.000&Aring;" />
 
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'''Structure of the E. coli PaaI protein from the phyenylacetic acid degradation operon'''<br />
 
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==Overview==
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==Structure of the E. coli PaaI protein from the phyenylacetic acid degradation operon==
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The structure and biochemical function of the hot dog-fold thioesterase PaaI operative in the aerobic phenylacetate degradation pathway are examined. PaaI showed modest activity with phenylacetyl-coenzyme A, suggestive of a role in coenzyme A release from this pathway intermediate in the event of limiting downstream pathway enzymes. Minimal activity was observed with aliphatic acyl-coenzyme A thioesters, which ruled out PaaI function in the lower phenylacetate pathway. PaaI was most active with ring-hydroxylated phenylacetyl-coenzyme A thioesters. The x-ray crystal structure of the Escherichia coli thioesterase is reported and analyzed to define the structural basis of substrate recognition and catalysis. The contributions of catalytic and substrate binding residues, thus, identified were examined through steady-state kinetic analysis of site-directed mutant proteins.
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<StructureSection load='2fs2' size='340' side='right'caption='[[2fs2]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FS2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FS2 FirstGlance]. <br>
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2FS2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FS2 OCA].
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fs2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fs2 OCA], [https://pdbe.org/2fs2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fs2 RCSB], [https://www.ebi.ac.uk/pdbsum/2fs2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fs2 ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/2fs2 TOPSAN]</span></td></tr>
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</table>
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==Reference==
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== Evolutionary Conservation ==
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Structure, function, and mechanism of the phenylacetate pathway hot dog-fold thioesterase PaaI., Song F, Zhuang Z, Finci L, Dunaway-Mariano D, Kniewel R, Buglino JA, Solorzano V, Wu J, Lima CD, J Biol Chem. 2006 Apr 21;281(16):11028-38. Epub 2006 Feb 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16464851 16464851]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Escherichia coli]]
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Check<jmol>
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[[Category: Single protein]]
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<jmolCheckbox>
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[[Category: Buglino, J A.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fs/2fs2_consurf.spt"</scriptWhenChecked>
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[[Category: Burley, S K.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: Kniewel, R.]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: Lima, C D.]]
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</jmolCheckbox>
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[[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fs2 ConSurf].
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[[Category: Solorzano, V.]]
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<div style="clear:both"></div>
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[[Category: Wu, J.]]
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__TOC__
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[[Category: SO4]]
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</StructureSection>
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[[Category: degradation]]
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[[Category: Large Structures]]
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[[Category: new york structural genomix research consortium]]
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[[Category: Buglino JA]]
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[[Category: nysgxrc]]
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[[Category: Burley SK]]
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[[Category: operon]]
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[[Category: Kniewel R]]
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[[Category: phenylacetic acid]]
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[[Category: Lima CD]]
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[[Category: protein structure initiative]]
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[[Category: Solorzano V]]
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[[Category: psi]]
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[[Category: Wu J]]
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[[Category: structural genomics]]
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[[Category: t820]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:24:31 2008''
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Current revision

Structure of the E. coli PaaI protein from the phyenylacetic acid degradation operon

PDB ID 2fs2

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