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2fx0
From Proteopedia
(Difference between revisions)
(New page: 200px<br /><applet load="2fx0" size="350" color="white" frame="true" align="right" spinBox="true" caption="2fx0, resolution 2.40Å" /> '''Crystal Structure of...) |
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| - | [[Image:2fx0.gif|left|200px]]<br /><applet load="2fx0" size="350" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="2fx0, resolution 2.40Å" /> | ||
| - | '''Crystal Structure of HlyIIR, a Hemolysin II transcriptional Regulator'''<br /> | ||
| - | == | + | ==Crystal Structure of HlyIIR, a Hemolysin II transcriptional Regulator== |
| - | Production of Bacillus cereus and Bacillus anthracis toxins is controlled | + | <StructureSection load='2fx0' size='340' side='right'caption='[[2fx0]], [[Resolution|resolution]] 2.40Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2fx0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_14579 Atcc 14579]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FX0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FX0 FirstGlance]. <br> | ||
| + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hlyIIR ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1396 ATCC 14579])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fx0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fx0 OCA], [https://pdbe.org/2fx0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fx0 RCSB], [https://www.ebi.ac.uk/pdbsum/2fx0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fx0 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fx/2fx0_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fx0 ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Production of Bacillus cereus and Bacillus anthracis toxins is controlled by a number of transcriptional regulators. Here we report the crystal structure of B. cereus HlyIIR, a regulator of the gene encoding the pore-forming toxin hemolysin II. We show that HlyIIR forms a tight dimer with a fold and overall architecture similar to the TetR family of repressors. A remarkable feature of the structure is a large internal cavity with a volume of 550 A(3) suggesting that the activity of HlyIIR is modulated by binding of a ligand, which triggers the toxin production. Virtual ligand library screening shows that this pocket can accommodate compounds with molecular masses of up to 400-500 Da. Based on structural data and previous biochemical evidence, we propose a model for HlyIIR interaction with the DNA. | ||
| - | + | Crystal structure of Bacillus cereus HlyIIR, a transcriptional regulator of the gene for pore-forming toxin hemolysin II.,Kovalevskiy OV, Lebedev AA, Surin AK, Solonin AS, Antson AA J Mol Biol. 2007 Jan 19;365(3):825-34. Epub 2006 Oct 26. PMID:17097673<ref>PMID:17097673</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | [[Category: | + | <div class="pdbe-citations 2fx0" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: Antson, A | + | <references/> |
| - | [[Category: Kovalevskiy, O | + | __TOC__ |
| - | [[Category: Lebedev, A | + | </StructureSection> |
| - | [[Category: Solonin, A | + | [[Category: Atcc 14579]] |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | + | [[Category: Antson, A A]] | |
| - | + | [[Category: Kovalevskiy, O V]] | |
| + | [[Category: Lebedev, A A]] | ||
| + | [[Category: Solonin, A S]] | ||
| + | [[Category: Transcription]] | ||
| + | [[Category: Transcriptional regulator]] | ||
Current revision
Crystal Structure of HlyIIR, a Hemolysin II transcriptional Regulator
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