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2jmh

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Revision as of 07:51, 7 April 2021

NMR solution structure of Blo t 5, a major mite allergen from Blomia tropicalis

Structural highlights

2jmh is a 1 chain structure with sequence from Blota. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	BLOT5 (BLOTA)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Blo t 5 is the major allergen from Blomia tropicalis mites and shows strong IgE reactivity with up to 90% of asthmatic and rhinitis patients' sera. The NMR solution structure of Blo t 5 comprises three long alpha helices, forming a coiled-coil, triple-helical bundle with a left-handed twist. TROSY-NMR experiments were used to study Blo t 5 interaction with the Fab' of a specific monoclonal antibody, mAb 4A7. The mAb epitope comprises two closely spaced surfaces, I and II, connected by a sharp turn and bearing critical residues Asn46 and Lys47 on one surface, and Lys54 and Arg57 on the other. This discontinuous epitope overlaps with the human IgE epitope(s) of Blo t 5. Epitope surface II is further predicted to undergo conformational exchange in the millisecond to microsecond range. The results presented are critical for the design of a hypoallergenic Blo t 5 for efficacious immunotherapy of allergic diseases.

Roles of structure and structural dynamics in the antibody recognition of the allergen proteins: an NMR study on Blomia tropicalis major allergen.,Naik MT, Chang CF, Kuo IC, Kung CC, Yi FC, Chua KY, Huang TH Structure. 2008 Jan;16(1):125-36. PMID:18184590^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Naik MT, Chang CF, Kuo IC, Kung CC, Yi FC, Chua KY, Huang TH. Roles of structure and structural dynamics in the antibody recognition of the allergen proteins: an NMR study on Blomia tropicalis major allergen. Structure. 2008 Jan;16(1):125-36. PMID:18184590 doi:10.1016/j.str.2007.10.022

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2jmh"

@@ Line 1: / Line 1: @@
 ==NMR solution structure of Blo t 5, a major mite allergen from Blomia tropicalis==
-<StructureSection load='2jmh' size='340' side='right' caption='[[2jmh]], [[NMR_Ensembles_of_Models | 21 NMR models]]' scene=''>
+<StructureSection load='2jmh' size='340' side='right'caption='[[2jmh]], [[NMR_Ensembles_of_Models | 21 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2jmh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Blota Blota]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JMH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JMH FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2jmh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Blota Blota]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JMH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JMH FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BLOT5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=40697 BLOTA])</td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BLOT5 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=40697 BLOTA])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jmh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jmh OCA], [http://pdbe.org/2jmh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jmh RCSB], [http://www.ebi.ac.uk/pdbsum/2jmh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jmh ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jmh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jmh OCA], [https://pdbe.org/2jmh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jmh RCSB], [https://www.ebi.ac.uk/pdbsum/2jmh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jmh ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 31: / Line 31: @@
 </StructureSection>
 [[Category: Blota]]
+[[Category: Large Structures]]
 [[Category: Chang, C]]
 [[Category: Chua, K]]

2jmh

From Proteopedia

Revision as of 07:51, 7 April 2021

NMR solution structure of Blo t 5, a major mite allergen from Blomia tropicalis

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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