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2k74

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{{Seed}}
 
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[[Image:2k74.png|left|200px]]
 
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==Solution NMR structure of DsbB-ubiquinone complex==
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The line below this paragraph, containing "STRUCTURE_2k74", creates the "Structure Box" on the page.
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<StructureSection load='2k74' size='340' side='right'caption='[[2k74]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2k74]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K74 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K74 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UQ2:UBIQUINONE-2'>UQ2</scene></td></tr>
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-->
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2hi7|2hi7]], [[2k73|2k73]]</div></td></tr>
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{{STRUCTURE_2k74| PDB=2k74 | SCENE= }}
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">dsbB, roxB, ycgA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k74 OCA], [https://pdbe.org/2k74 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k74 RCSB], [https://www.ebi.ac.uk/pdbsum/2k74 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k74 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/DSBB_ECOLI DSBB_ECOLI]] Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by oxidizing the DsbA protein.<ref>PMID:8430071</ref> <ref>PMID:7688471</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k7/2k74_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2k74 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We describe the NMR structure of DsbB, a polytopic helical membrane protein. DsbB, a bacterial cytoplasmic membrane protein, plays a key role in disulfide bond formation. It reoxidizes DsbA, the periplasmic protein disulfide oxidant, using the oxidizing power of membrane-embedded quinones. We determined the structure of an interloop disulfide bond form of DsbB, an intermediate in catalysis. Analysis of the structure and interactions with substrates DsbA and quinone reveals functionally relevant changes induced by these substrates. Analysis of the structure, dynamics measurements, and NMR chemical shifts around the interloop disulfide bond suggest how electron movement from DsbA to quinone through DsbB is regulated and facilitated. Our results demonstrate the extraordinary utility of NMR for functional characterization of polytopic integral membrane proteins and provide insights into the mechanism of DsbB catalysis.
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===Solution NMR structure of DsbB-ubiquinone complex===
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NMR solution structure of the integral membrane enzyme DsbB: functional insights into DsbB-catalyzed disulfide bond formation.,Zhou Y, Cierpicki T, Jimenez RH, Lukasik SM, Ellena JF, Cafiso DS, Kadokura H, Beckwith J, Bushweller JH Mol Cell. 2008 Sep 26;31(6):896-908. PMID:18922471<ref>PMID:18922471</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2k74" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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2K74 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k12 Escherichia coli k12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K74 OCA].
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*[[Thiol:disulfide interchange protein 3D structures|Thiol:disulfide interchange protein 3D structures]]
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[[Category: Escherichia coli k12]]
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== References ==
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[[Category: Beckwith, J.]]
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<references/>
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[[Category: Bushweller, J H.]]
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__TOC__
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[[Category: Cafiso, D S.]]
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</StructureSection>
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[[Category: Cierpicki, T.]]
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[[Category: Ecoli]]
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[[Category: Ellena, J F.]]
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[[Category: Large Structures]]
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[[Category: Jimenez, R H.Flores.]]
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[[Category: Beckwith, J]]
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[[Category: Kadokura, H.]]
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[[Category: Bushweller, J H]]
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[[Category: Lukasik, S M.]]
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[[Category: Cafiso, D S]]
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[[Category: Zhou, Y.]]
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[[Category: Cierpicki, T]]
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[[Category: Ellena, J F]]
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[[Category: Jimenez, R H.Flores]]
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[[Category: Kadokura, H]]
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[[Category: Lukasik, S M]]
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[[Category: Zhou, Y]]
[[Category: Chaperone]]
[[Category: Chaperone]]
[[Category: Disulfide bond]]
[[Category: Disulfide bond]]
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[[Category: Transport]]
[[Category: Transport]]
[[Category: Ubiquinone]]
[[Category: Ubiquinone]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 17:12:12 2009''
 

Current revision

Solution NMR structure of DsbB-ubiquinone complex

PDB ID 2k74

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