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1e5t

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[[Image:1e5t.jpg|left|200px]]
[[Image:1e5t.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1e5t |SIZE=350|CAPTION= <scene name='initialview01'>1e5t</scene>, resolution 1.7&Aring;
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The line below this paragraph, containing "STRUCTURE_1e5t", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AS:Active+Site,+Catalytic+Triad'>AS</scene> and <scene name='pdbsite=SS:Disulfide+Engineered+Between+Cys78+And+Gln397cys'>SS</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Prolyl_oligopeptidase Prolyl oligopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.26 3.4.21.26] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1e5t| PDB=1e5t | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e5t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e5t OCA], [http://www.ebi.ac.uk/pdbsum/1e5t PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1e5t RCSB]</span>
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}}
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'''PROLYL OLIGOPEPTIDASE FROM PORCINE BRAIN, MUTANT'''
'''PROLYL OLIGOPEPTIDASE FROM PORCINE BRAIN, MUTANT'''
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[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
[[Category: Fulop, V.]]
[[Category: Fulop, V.]]
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[[Category: alpha/ beta-hydrolase]]
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[[Category: Alpha/ beta-hydrolase]]
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[[Category: amnesia]]
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[[Category: Amnesia]]
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[[Category: beta-propeller]]
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[[Category: Beta-propeller]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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[[Category: prolyl oligopeptidase]]
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[[Category: Prolyl oligopeptidase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:42:07 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:55:44 2008''
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Revision as of 11:42, 2 May 2008

Image:1e5t.jpg

Template:STRUCTURE 1e5t

PROLYL OLIGOPEPTIDASE FROM PORCINE BRAIN, MUTANT


Overview

Proteases have a variety of strategies for selecting substrates in order to prevent uncontrolled protein degradation. A recent crystal structure determination of prolyl oligopeptidase has suggested a way for substrate selection involving an unclosed seven-bladed beta-propeller domain. We have engineered a disulfide bond between the first and seventh blades of the propeller, which resulted in the loss of enzymatic activity. These results provided direct evidence for a novel strategy of regulation in which oscillating propeller blades act as a gating filter during catalysis, letting small peptide substrates into the active site while excluding large proteins to prevent accidental proteolysis.

About this Structure

1E5T is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.

Reference

Catalysis of serine oligopeptidases is controlled by a gating filter mechanism., Fulop V, Szeltner Z, Polgar L, EMBO Rep. 2000 Sep;1(3):277-81. PMID:11256612 Page seeded by OCA on Fri May 2 14:42:07 2008

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