1eb3

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[[Image:1eb3.jpg|left|200px]]
[[Image:1eb3.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1eb3 |SIZE=350|CAPTION= <scene name='initialview01'>1eb3</scene>, resolution 1.75&Aring;
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The line below this paragraph, containing "STRUCTURE_1eb3", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Dsb+Binding+Site+For+Chain+A'>AC1</scene> and <scene name='pdbsite=AC2:Zn+Binding+Site+For+Chain+A'>AC2</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DSB:4,7-DIOXOSEBACIC+ACID'>DSB</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Porphobilinogen_synthase Porphobilinogen synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.24 4.2.1.24] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1eb3| PDB=1eb3 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eb3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eb3 OCA], [http://www.ebi.ac.uk/pdbsum/1eb3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1eb3 RCSB]</span>
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}}
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'''YEAST 5-AMINOLAEVULINIC ACID DEHYDRATASE 4,7-DIOXOSEBACIC ACID COMPLEX'''
'''YEAST 5-AMINOLAEVULINIC ACID DEHYDRATASE 4,7-DIOXOSEBACIC ACID COMPLEX'''
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[[Category: Warren, M J.]]
[[Category: Warren, M J.]]
[[Category: Wood, S P.]]
[[Category: Wood, S P.]]
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[[Category: aldolase]]
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[[Category: Aldolase]]
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[[Category: dehydratase]]
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[[Category: Dehydratase]]
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[[Category: tetrapyrrole synthesis]]
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[[Category: Tetrapyrrole synthesis]]
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[[Category: tim barrel]]
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[[Category: Tim barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:53:31 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:59:04 2008''
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Revision as of 11:53, 2 May 2008

Image:1eb3.jpg

Template:STRUCTURE 1eb3

YEAST 5-AMINOLAEVULINIC ACID DEHYDRATASE 4,7-DIOXOSEBACIC ACID COMPLEX


Overview

The structures of 5-aminolaevulinic acid dehydratase complexed with two irreversible inhibitors (4-oxosebacic acid and 4,7-dioxosebacic acid) have been solved at high resolution. Both inhibitors bind by forming a Schiff base link with Lys 263 at the active site. Previous inhibitor binding studies have defined the interactions made by only one of the two substrate moieties (P-side substrate) which bind to the enzyme during catalysis. The structures reported here provide an improved definition of the interactions made by both of the substrate molecules (A- and P-side substrates). The most intriguing result is the novel finding that 4,7-dioxosebacic acid forms a second Schiff base with the enzyme involving Lys 210. It has been known for many years that P-side substrate forms a Schiff base (with Lys 263) but until now there has been no evidence that binding of A-side substrate involves formation of a Schiff base with the enzyme. A catalytic mechanism involving substrate linked to the enzyme through Schiff bases at both the A- and P-sites is proposed.

About this Structure

1EB3 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

The X-ray structure of yeast 5-aminolaevulinic acid dehydratase complexed with two diacid inhibitors., Erskine PT, Coates L, Newbold R, Brindley AA, Stauffer F, Wood SP, Warren MJ, Cooper JB, Shoolingin-Jordan PM, Neier R, FEBS Lett. 2001 Aug 17;503(2-3):196-200. PMID:11513881 Page seeded by OCA on Fri May 2 14:53:31 2008

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OCA

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