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2ga4

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(New page: 200px<br /><applet load="2ga4" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ga4, resolution 1.800&Aring;" /> '''Stx2 with adenine''...)
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[[Image:2ga4.gif|left|200px]]<br /><applet load="2ga4" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2ga4, resolution 1.800&Aring;" />
 
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'''Stx2 with adenine'''<br />
 
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==Overview==
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==Stx2 with adenine==
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Stx2 is a protein toxin whose catalytic subunit acts as an N-glycosidase, to depurinate a specific adenine base from 28S rRNA. In the holotoxin, the, catalytic portion, A1, is linked to the rest of the A subunit, A2, and A2, interacts with the pentameric ring formed by the five B subunits. In order, to test whether the holotoxin is active as an N-glycosidase, Stx2 was, crystallized in the presence of adenosine and adenine. The crystals, diffracted to approximately 1.8 angstroms and showed clear electron, density for adenine in the active site. Adenosine had been cleaved, proving that Stx2 is an active N-glycosidase. While the holotoxin is, active against small substrates, it would be expected that the B subunits, would interfere with the binding of the 28S rRNA.
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<StructureSection load='2ga4' size='340' side='right'caption='[[2ga4]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2ga4]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bp933 Bp933]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GA4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GA4 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PS:3-PYRIDINIUM-1-YLPROPANE-1-SULFONATE'>1PS</scene>, <scene name='pdbligand=ADE:ADENINE'>ADE</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1r4p|1r4p]], [[1r4q|1r4q]]</div></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ga4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ga4 OCA], [https://pdbe.org/2ga4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ga4 RCSB], [https://www.ebi.ac.uk/pdbsum/2ga4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ga4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/STXA_BP933 STXA_BP933]] The A subunit is responsible for inhibiting protein synthesis through the catalytic inactivation of 60S ribosomal subunits. After endocytosis, the A subunit is cleaved by furin in two fragments, A1 and A2: A1 is the catalytically active fragment, and A2 is essential for holotoxin assembly with the B subunits. [[https://www.uniprot.org/uniprot/STXB_BP933 STXB_BP933]] The B subunit is responsible for the binding of the holotoxin to specific receptors on the target cell surface, such as globotriaosylceramide (Gb3) in human intestinal microvilli.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ga/2ga4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ga4 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Stx2 is a protein toxin whose catalytic subunit acts as an N-glycosidase to depurinate a specific adenine base from 28S rRNA. In the holotoxin, the catalytic portion, A1, is linked to the rest of the A subunit, A2, and A2 interacts with the pentameric ring formed by the five B subunits. In order to test whether the holotoxin is active as an N-glycosidase, Stx2 was crystallized in the presence of adenosine and adenine. The crystals diffracted to approximately 1.8 angstroms and showed clear electron density for adenine in the active site. Adenosine had been cleaved, proving that Stx2 is an active N-glycosidase. While the holotoxin is active against small substrates, it would be expected that the B subunits would interfere with the binding of the 28S rRNA.
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==About this Structure==
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Binding of adenine to Stx2, the protein toxin from Escherichia coli O157:H7.,Fraser ME, Cherney MM, Marcato P, Mulvey GL, Armstrong GD, James MN Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Jul 1;62(Pt, 7):627-30. Epub 2006 Jun 26. PMID:16820678<ref>PMID:16820678</ref>
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2GA4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacteriophage_933w Bacteriophage 933w] with NA, ADE, 1PS, EDO and FMT as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2GA4 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Binding of adenine to Stx2, the protein toxin from Escherichia coli O157:H7., Fraser ME, Cherney MM, Marcato P, Mulvey GL, Armstrong GD, James MN, Acta Crystallograph Sect F Struct Biol Cryst Commun. 2006 Jul 1;62(Pt, 7):627-30. Epub 2006 Jun 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16820678 16820678]
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</div>
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[[Category: Bacteriophage 933w]]
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<div class="pdbe-citations 2ga4" style="background-color:#fffaf0;"></div>
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[[Category: Protein complex]]
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[[Category: rRNA N-glycosylase]]
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[[Category: Fraser, M.E.]]
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[[Category: 1PS]]
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[[Category: ADE]]
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[[Category: EDO]]
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[[Category: FMT]]
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[[Category: NA]]
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[[Category: ab5-toxin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:04:17 2007''
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==See Also==
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*[[Shiga toxin|Shiga toxin]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Bp933]]
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[[Category: Large Structures]]
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[[Category: RRNA N-glycosylase]]
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[[Category: Fraser, M E]]
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[[Category: Ab5-toxin]]
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[[Category: Toxin]]

Current revision

Stx2 with adenine

PDB ID 2ga4

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