2hoi

From Proteopedia

(Difference between revisions)

Revision as of 10:03, 12 May 2021

Crystal structure of the tetrameric pre-cleavage synaptic complex in the cre-loxp site-specific recombination

Structural highlights

2hoi is a 8 chain structure with sequence from Bpp1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1crx, 4crx, 2hof
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RECR_BPP1] Catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Cre recombinase catalyzes site-specific recombination between 34-bp loxP sites in a variety of topological and cellular contexts. An obligatory step in the recombination reaction is the association, or synapsis, of Cre-bound loxP sites to form a tetrameric protein assembly that is competent for strand exchange. Using analytical ultracentrifugation and electrophoresis approaches, we have studied the energetics of Cre-mediated synapsis of loxP sites. We found that synapsis occurs with a high affinity (Kd = 10 nM) and is pH-dependent but does not require divalent cations. Surprisingly, the catalytically inactive Cre K201A mutant is fully competent for synapsis of loxP sites, yet the inactive Y324F and R173K mutants are defective for synapsis. These findings have allowed us to determine the first crystal structures of a pre-cleavage Cre-loxP synaptic complex in a configuration representing the starting point in the recombination pathway. When combined with a quantitative analysis of synapsis using loxP mutants, the structures explain how the central 8 bp of the loxP site are able to dictate the order of strand exchange in the Cre system.

Synapsis of loxP sites by Cre recombinase.,Ghosh K, Guo F, Van Duyne GD J Biol Chem. 2007 Aug 17;282(33):24004-16. Epub 2007 Jun 15. PMID:17573343^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ghosh K, Guo F, Van Duyne GD. Synapsis of loxP sites by Cre recombinase. J Biol Chem. 2007 Aug 17;282(33):24004-16. Epub 2007 Jun 15. PMID:17573343 doi:10.1074/jbc.M703283200

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2hoi"

@@ Line 3: / Line 3: @@
 <StructureSection load='2hoi' size='340' side='right'caption='[[2hoi]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2hoi]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpp1 Bpp1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HOI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HOI FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2hoi]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Bpp1 Bpp1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HOI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HOI FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1crx|1crx]], [[4crx|4crx]], [[2hof|2hof]]</td></tr>
+</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1crx|1crx]], [[4crx|4crx]], [[2hof|2hof]]</div></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hoi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hoi OCA], [http://pdbe.org/2hoi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hoi RCSB], [http://www.ebi.ac.uk/pdbsum/2hoi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2hoi ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hoi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hoi OCA], [https://pdbe.org/2hoi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hoi RCSB], [https://www.ebi.ac.uk/pdbsum/2hoi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hoi ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/RECR_BPP1 RECR_BPP1]] Catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state.
+[[https://www.uniprot.org/uniprot/RECR_BPP1 RECR_BPP1]] Catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 30: / Line 30: @@
 ==See Also==
-*[[Resolvase|Resolvase]]
+*[[Resolvase 3D structures|Resolvase 3D structures]]
 == References ==
 <references/>

2hoi

From Proteopedia

Revision as of 10:03, 12 May 2021

Crystal structure of the tetrameric pre-cleavage synaptic complex in the cre-loxp site-specific recombination

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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