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2liy

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Revision as of 10:11, 12 May 2021

Plant peptide hormone regulating stomatal density

Structural highlights

2liy is a 1 chain structure with sequence from Arath. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	EPFL9 (ARATH)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[EPFL9_ARATH] Controls stomatal patterning (By similarity).

Publication Abstract from PubMed

Stomatal development in plants is regulated by defensin-like secretory epidermal patterning factor (EPF) peptide hormones. Only one of these, stomagen, is a positive regulator, whereas EPF1, EPF2, and possibly others are negative regulators. Here we explore the structure-function relationships of EPFs, by integrating NMR and semi-in vitro stomagen experiments. We show that stomagen is composed of a loop and a scaffold containing three disulphide bonds. A mutant composed of the stomagen loop and the EPF2 scaffold positively regulates the stomatal density on Arabidopsis cotyledons. The reciprocal mutant composed of the EPF2 loop and the stomagen scaffold acts negatively. Deletion of the disulphide bond introduces unfolding and inactivity. Our results suggest that the loop confers the functional specificity of EPFs and that the scaffold is structurally required for their activity. This structural decomposition approach to elucidating the functional site could be adapted for the analysis of other cysteine-rich peptide families.

The NMR structure of stomagen reveals the basis of stomatal density regulation by plant peptide hormones.,Ohki S, Takeuchi M, Mori M Nat Commun. 2011 Oct 25;2:512. doi: 10.1038/ncomms1520. PMID:22027592^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ohki S, Takeuchi M, Mori M. The NMR structure of stomagen reveals the basis of stomatal density regulation by plant peptide hormones. Nat Commun. 2011 Oct 25;2:512. doi: 10.1038/ncomms1520. PMID:22027592 doi:http://dx.doi.org/10.1038/ncomms1520

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2liy"

@@ Line 1: / Line 1: @@
 ==Plant peptide hormone regulating stomatal density==
-<StructureSection load='2liy' size='340' side='right' caption='[[2liy]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2liy' size='340' side='right'caption='[[2liy]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2liy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LIY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LIY FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2liy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arath Arath]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LIY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LIY FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">EPFL9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">EPFL9 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2liy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2liy OCA], [http://pdbe.org/2liy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2liy RCSB], [http://www.ebi.ac.uk/pdbsum/2liy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2liy ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2liy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2liy OCA], [https://pdbe.org/2liy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2liy RCSB], [https://www.ebi.ac.uk/pdbsum/2liy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2liy ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/EPFL9_ARATH EPFL9_ARATH]] Controls stomatal patterning (By similarity).
+[[https://www.uniprot.org/uniprot/EPFL9_ARATH EPFL9_ARATH]] Controls stomatal patterning (By similarity).
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 23: @@
 </StructureSection>
 [[Category: Arath]]
+[[Category: Large Structures]]
 [[Category: Ohki, S]]
 [[Category: Epfl family]]

2liy

From Proteopedia

Revision as of 10:11, 12 May 2021

Plant peptide hormone regulating stomatal density

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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