1ej5
From Proteopedia
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'''SOLUTION STRUCTURE OF THE AUTOINHIBITED CONFORMATION OF WASP''' | '''SOLUTION STRUCTURE OF THE AUTOINHIBITED CONFORMATION OF WASP''' | ||
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[[Category: Liu, G A.]] | [[Category: Liu, G A.]] | ||
[[Category: Rosen, M K.]] | [[Category: Rosen, M K.]] | ||
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| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:09:50 2008'' | |
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Revision as of 12:09, 2 May 2008
SOLUTION STRUCTURE OF THE AUTOINHIBITED CONFORMATION OF WASP
Overview
The Rho-family GTPase, Cdc42, can regulate the actin cytoskeleton through activation of Wiskott-Aldrich syndrome protein (WASP) family members. Activation relieves an autoinhibitory contact between the GTPase-binding domain and the carboxy-terminal region of WASP proteins. Here we report the autoinhibited structure of the GTPase-binding domain of WASP, which can be induced by the C-terminal region or by organic co-solvents. In the autoinhibited complex, intramolecular interactions with the GTPase-binding domain occlude residues of the C terminus that regulate the Arp2/3 actin-nucleating complex. Binding of Cdc42 to the GTPase-binding domain causes a dramatic conformational change, resulting in disruption of the hydrophobic core and release of the C terminus, enabling its interaction with the actin regulatory machinery. These data show that 'intrinsically unstructured' peptides such as the GTPase-binding domain of WASP can be induced into distinct structural and functional states depending on context.
About this Structure
1EJ5 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Autoinhibition and activation mechanisms of the Wiskott-Aldrich syndrome protein., Kim AS, Kakalis LT, Abdul-Manan N, Liu GA, Rosen MK, Nature. 2000 Mar 9;404(6774):151-8. PMID:10724160 Page seeded by OCA on Fri May 2 15:09:50 2008
