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2mnj

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Revision as of 15:17, 2 June 2021

NMR solution structure of the yeast Pih1 and Tah1 C-terminal domains complex

Structural highlights

2mnj is a 2 chain structure with sequence from Atcc 18824. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	TAH1, YCR060W, YCR60W (ATCC 18824), PIH1, NOP17, YHR034C (ATCC 18824)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PIH1_YEAST] Involved in pre-rRNA processing and required for the NOP58-snoRNA interaction.^[1]

Publication Abstract from PubMed

In eukaryotes, nucleotide post-transcriptional modifications in RNAs play an essential role in cell proliferation by contributing to pre-ribosomal RNA processing, ribosome assembly and activity. Box C/D small nucleolar ribonucleoparticles catalyze site-specific 2'-O-methylation of riboses, one of the most prevalent RNA modifications. They contain one guide RNA and four core proteins and their in vivo assembly requires numerous factors including (HUMAN/Yeast) BCD1/Bcd1p, NUFIP1/Rsa1p, ZNHIT3/Hit1p, the R2TP complex composed of protein PIH1D1/Pih1p and RPAP3/Tah1p that bridges the R2TP complex to the HSP90/Hsp82 chaperone and two AAA+ ATPases. We show that Tah1p can stabilize Pih1p in the absence of Hsp82 activity during the stationary phase of growth and consequently that the Tah1p:Pih1p interaction is sufficient for Pih1p stability. This prompted us to establish the solution structure of the Tah1p:Pih1p complex by NMR. The C-terminal tail S93-S111 of Tah1p snakes along Pih1p264-344 folded in a CS domain to form two intermolecular beta-sheets and one covering loop. However, a thorough inspection of the NMR and crystal structures revealed structural differences that may be of functional importance. In addition, our NMR and isothermal titration calorimetry data revealed the formation of direct contacts between Pih1p257-344 and the Hsp82MC domain in the presence of Tah1p. By co-expression in Escherichiacoli, we demonstrate that Pih1p has two other direct partners, the Rsa1p assembly factor and the Nop58p core protein, and in vivo and in vitro experiments mapped the required binding domains. Our data suggest that these two interactions are mutually exclusive. The implication of this finding for box C/D small nucleolar ribonucleoparticle assembly is discussed.

Structure/Function Analysis of Protein-Protein Interactions Developed by the Yeast Pih1 Platform Protein and Its Partners in Box C/D snoRNP Assembly.,Quinternet M, Rothe B, Barbier M, Bobo C, Saliou JM, Jacquemin C, Back R, Chagot ME, Cianferani S, Meyer P, Branlant C, Charpentier B, Manival X J Mol Biol. 2015 Jul 22. pii: S0022-2836(15)00393-9. doi:, 10.1016/j.jmb.2015.07.012. PMID:26210662^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Gonzales FA, Zanchin NI, Luz JS, Oliveira CC. Characterization of Saccharomyces cerevisiae Nop17p, a novel Nop58p-interacting protein that is involved in Pre-rRNA processing. J Mol Biol. 2005 Feb 18;346(2):437-55. Epub 2004 Dec 21. PMID:15670595 doi:http://dx.doi.org/S0022-2836(04)01552-9
↑ Quinternet M, Rothe B, Barbier M, Bobo C, Saliou JM, Jacquemin C, Back R, Chagot ME, Cianferani S, Meyer P, Branlant C, Charpentier B, Manival X. Structure/Function Analysis of Protein-Protein Interactions Developed by the Yeast Pih1 Platform Protein and Its Partners in Box C/D snoRNP Assembly. J Mol Biol. 2015 Jul 22. pii: S0022-2836(15)00393-9. doi:, 10.1016/j.jmb.2015.07.012. PMID:26210662 doi:http://dx.doi.org/10.1016/j.jmb.2015.07.012

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2mnj"

@@ Line 1: / Line 1: @@
 ==NMR solution structure of the yeast Pih1 and Tah1 C-terminal domains complex==
-<StructureSection load='2mnj' size='340' side='right' caption='[[2mnj]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2mnj' size='340' side='right'caption='[[2mnj]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2mnj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MNJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MNJ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2mnj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MNJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MNJ FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TAH1, YCR060W, YCR60W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), PIH1, NOP17, YHR034C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TAH1, YCR060W, YCR60W ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), PIH1, NOP17, YHR034C ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mnj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mnj OCA], [http://pdbe.org/2mnj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mnj RCSB], [http://www.ebi.ac.uk/pdbsum/2mnj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2mnj ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mnj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mnj OCA], [https://pdbe.org/2mnj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mnj RCSB], [https://www.ebi.ac.uk/pdbsum/2mnj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mnj ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/PIH1_YEAST PIH1_YEAST]] Involved in pre-rRNA processing and required for the NOP58-snoRNA interaction.<ref>PMID:15670595</ref>
+[[https://www.uniprot.org/uniprot/PIH1_YEAST PIH1_YEAST]] Involved in pre-rRNA processing and required for the NOP58-snoRNA interaction.<ref>PMID:15670595</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 23: @@
 </StructureSection>
 [[Category: Atcc 18824]]
+[[Category: Large Structures]]
 [[Category: Charpentier, B]]
 [[Category: Jacquemin, C]]

2mnj

From Proteopedia

Revision as of 15:17, 2 June 2021

NMR solution structure of the yeast Pih1 and Tah1 C-terminal domains complex

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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