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| | ==Crystal structure of Spc42p, a critical component of spindle pole body in budding yeast== | | ==Crystal structure of Spc42p, a critical component of spindle pole body in budding yeast== |
| - | <StructureSection load='2q6q' size='340' side='right' caption='[[2q6q]], [[Resolution|resolution]] 1.97Å' scene=''> | + | <StructureSection load='2q6q' size='340' side='right'caption='[[2q6q]], [[Resolution|resolution]] 1.97Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2q6q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q6Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2Q6Q FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2q6q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q6Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Q6Q FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SPC42 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr> | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SPC42 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2q6q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q6q OCA], [http://pdbe.org/2q6q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2q6q RCSB], [http://www.ebi.ac.uk/pdbsum/2q6q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2q6q ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2q6q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q6q OCA], [https://pdbe.org/2q6q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2q6q RCSB], [https://www.ebi.ac.uk/pdbsum/2q6q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2q6q ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SPC42_YEAST SPC42_YEAST]] Forms a polymeric layer at the periphery of the spindle pole body (SPB) central plaque which has an essential function during SPB duplication and may facilitate attachment of the SPB to the nuclear membrane.<ref>PMID:10339566</ref> | + | [[https://www.uniprot.org/uniprot/SPC42_YEAST SPC42_YEAST]] Forms a polymeric layer at the periphery of the spindle pole body (SPB) central plaque which has an essential function during SPB duplication and may facilitate attachment of the SPB to the nuclear membrane.<ref>PMID:10339566</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Atcc 18824]] | | [[Category: Atcc 18824]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Kim, P S]] | | [[Category: Kim, P S]] |
| | [[Category: Malashkevich, V N]] | | [[Category: Malashkevich, V N]] |
| Structural highlights
Function
[SPC42_YEAST] Forms a polymeric layer at the periphery of the spindle pole body (SPB) central plaque which has an essential function during SPB duplication and may facilitate attachment of the SPB to the nuclear membrane.[1]
Publication Abstract from PubMed
The spindle pole body (SPB) is a multiprotein complex that organizes microtubules in yeast. Due to its large size and association with the nuclear membrane, little is known about its detailed structure. In particular, although many SPB components and some of the interactions between them have been identified, the molecular details of how most of these interactions occur are not known. The prevalence of predicted coiled-coil regions in SPB proteins suggests that some interactions may occur via coiled coils. Here this hypothesis is supported by biochemical characterization of isolated coiled-coil peptides derived from SPB proteins. Formation of four strongly self-associating coiled-coil complexes from Spc29, Spc42, and Spc72 was demonstrated by circular dichroism (CD) spectroscopy and a fluorescence resonance energy transfer (FRET) assay. Many weaker self- and heteroassociations were also detected by CD, FRET, and/or cross-linking. The thermal stabilities of nine candidate homooligomers were assessed; six unfolded cooperatively with melting temperatures ranging from <11 to >50 degrees C. Solution studies established that coiled-coil peptides derived from Spc42 and Spc72 form parallel dimers, and this was confirmed for Spc42 by a high-resolution crystal structure. These data contribute to a growing body of knowledge that will ultimately provide a detailed model of the SPB structure.
Analysis of coiled-coil interactions between core proteins of the spindle pole body.,Zizlsperger N, Malashkevich VN, Pillay S, Keating AE Biochemistry. 2008 Nov 11;47(45):11858-68. Epub 2008 Oct 14. PMID:18850724[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Elliott S, Knop M, Schlenstedt G, Schiebel E. Spc29p is a component of the Spc110p subcomplex and is essential for spindle pole body duplication. Proc Natl Acad Sci U S A. 1999 May 25;96(11):6205-10. PMID:10339566
- ↑ Zizlsperger N, Malashkevich VN, Pillay S, Keating AE. Analysis of coiled-coil interactions between core proteins of the spindle pole body. Biochemistry. 2008 Nov 11;47(45):11858-68. Epub 2008 Oct 14. PMID:18850724 doi:10.1021/bi801378z
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