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2qo3

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{{Seed}}
 
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[[Image:2qo3.png|left|200px]]
 
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==Crystal Structure of [KS3][AT3] didomain from module 3 of 6-deoxyerthronolide B synthase==
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The line below this paragraph, containing "STRUCTURE_2qo3", creates the "Structure Box" on the page.
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<StructureSection load='2qo3' size='340' side='right'caption='[[2qo3]], [[Resolution|resolution]] 2.59&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2qo3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"actinomyces_erythreus"_(sic)_waksman_1923 "actinomyces erythreus" (sic) waksman 1923]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QO3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QO3 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CER:(2S,+3R)-3-HYDROXY-4-OXO-7,10-TRANS,TRANS-DODECADIENAMIDE'>CER</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2hg4|2hg4]]</div></td></tr>
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{{STRUCTURE_2qo3| PDB=2qo3 | SCENE= }}
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">eryAII ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1836 "Actinomyces erythreus" (sic) Waksman 1923])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/6-deoxyerythronolide-B_synthase 6-deoxyerythronolide-B synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.94 2.3.1.94] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qo3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qo3 OCA], [https://pdbe.org/2qo3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qo3 RCSB], [https://www.ebi.ac.uk/pdbsum/2qo3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qo3 ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qo/2qo3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qo3 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We report the 2.6 A X-ray crystal structure of a 190 kDa homodimeric fragment from module 3 of the 6-deoxyerthronolide B synthase covalently bound to the inhibitor cerulenin. The structure shows two well-organized interdomain linker regions in addition to the full-length ketosynthase (KS) and acyltransferase (AT) domains. Analysis of the substrate-binding site of the KS domain suggests that a loop region at the homodimer interface influences KS substrate specificity. We also describe a model for the interaction of the catalytic domains with the acyl carrier protein (ACP) domain. The ACP is proposed to dock within a deep cleft between the KS and AT domains, with interactions that span both the KS homodimer and AT domain. In conjunction with other recent data, our results provide atomic resolution pictures of several catalytically relevant protein interactions in this remarkable family of modular megasynthases.
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===Crystal Structure of [KS3][AT3] didomain from module 3 of 6-deoxyerthronolide B synthase===
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Structural and mechanistic analysis of protein interactions in module 3 of the 6-deoxyerythronolide B synthase.,Tang Y, Chen AY, Kim CY, Cane DE, Khosla C Chem Biol. 2007 Aug;14(8):931-43. PMID:17719492<ref>PMID:17719492</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2qo3" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_17719492}}, adds the Publication Abstract to the page
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*[[6-deoxyerythronolide B synthase 3D structures|6-deoxyerythronolide B synthase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 17719492 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17719492}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: 6-deoxyerythronolide-B synthase]]
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2QO3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharopolyspora_erythraea Saccharopolyspora erythraea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QO3 OCA].
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[[Category: Large Structures]]
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[[Category: Cane, E D]]
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==Reference==
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[[Category: Chen, Y A]]
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Structural and mechanistic analysis of protein interactions in module 3 of the 6-deoxyerythronolide B synthase., Tang Y, Chen AY, Kim CY, Cane DE, Khosla C, Chem Biol. 2007 Aug;14(8):931-43. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17719492 17719492]
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[[Category: Khosla, C]]
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[[Category: Kim, C Y]]
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Structure-based dissociation of a type I polyketide synthase module., Chen AY, Cane DE, Khosla C, Chem Biol. 2007 Jul;14(7):784-92. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17656315 17656315]
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[[Category: Tang, Y]]
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The 2.7-Angstrom crystal structure of a 194-kDa homodimeric fragment of the 6-deoxyerythronolide B synthase., Tang Y, Kim CY, Mathews II, Cane DE, Khosla C, Proc Natl Acad Sci U S A. 2006 Jul 25;103(30):11124-9. Epub 2006 Jul 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16844787 16844787]
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Reconstituting modular activity from separated domains of 6-deoxyerythronolide B synthase., Kim CY, Alekseyev VY, Chen AY, Tang Y, Cane DE, Khosla C, Biochemistry. 2004 Nov 9;43(44):13892-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15518537 15518537]
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[[Category: Erythronolide synthase]]
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[[Category: Saccharopolyspora erythraea]]
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[[Category: Single protein]]
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[[Category: Cane, E D.]]
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[[Category: Chen, Y A.]]
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[[Category: Khosla, C.]]
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[[Category: Kim, C Y.]]
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[[Category: Tang, Y.]]
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[[Category: Acyltransferase]]
[[Category: Acyltransferase]]
[[Category: Ketosynthase]]
[[Category: Ketosynthase]]
[[Category: Phosphopantetheine]]
[[Category: Phosphopantetheine]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:41:34 2008''
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Current revision

Crystal Structure of [KS3][AT3] didomain from module 3 of 6-deoxyerthronolide B synthase

PDB ID 2qo3

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